Table 1

Overview of the two microarray platforms and TaqMan® GeneExpression Assay based real-time PCR

Applied Biosystems

Agilent

TaqMan Gene Expression Assays


Platforms

Human Genome Survey Microarray

Human Whole Genome Arrays

TaqMan Expression Assays

Technology

Hybridization

Comparative Hybridization

5' Nuclease Chemistry & Real Time PCR

Probe (bases)

60mer

60mer

TaqMan Primer & Probes

Substrate

Nylon

Glass slide

-

Deposition

Contact Spotting

In-situ Ink Jet Printing

-

Detection

One-color Chemiluminescence

Two-color Cy3/Cy5 Fluorescence

One-color FAM Fluorescence

Software

1700 Chemiluminescence Microaray Analyzer Software v 1.1

Feature Extraction A 7.5.1

SDS 2.1

Total Probes

33,096 probes

44,000 probes

1375 Selected Targets


Wang et al. BMC Genomics 2006 7:59   doi:10.1186/1471-2164-7-59

Open Data