Additional File 1.
Scheme of TaqMan® Gene Expression Assay Based Real-time PCR. A TaqMan probe is designed to anneal to the target sequence between the traditional forward and reverse PCR primers. A fluorescent reporter dye and a quencher moiety are attached to the 5' and 3' ends of this TaqMan probe and when the probe is intact, the reporter dye emission is quenched. During each PCR extension cycle, the AmpliTaq Gold® DNA polymerase has an intrinsic 5' to 3' nuclease activity and cleaves the reporter dye from the probe. Once separated from the quencher, the reporter dye emits its characteristic fluorescence. Because the amount of fluorescent signal released during each cycle of amplification is proportional to the amount of product generated, this provides the basis for the quantitative measurements of gene expressions.
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Wang et al. BMC Genomics 2006 7:59 doi:10.1186/1471-2164-7-59