Correlation of fold change in pair-wise tissues determined by microarray platforms and TaqMan® Gene Expression Assay based real-time PCR. y-axis, fold change determined by microarrays which is defined as: For Applied Biosystems Arrays, log2 (MedianSignal_tissue1/MedianSignal_tissue2); for Agilent arrays, log2(MedianSignal_tissue1/MedianSignal_UHR)- log2(medianSignal_tissue2/MedianSignal_UHR); x-axis, fold change determined by real-time PCR, which is defined as ΔΔCt = (Ct_tissue2-Ct_PPIA)-(Ct_tissue1-Ct_PPIA). For each pair-wise comparison, genes were filtered based on real-time PCR detection thresholds (detectable in at least 3 out of 4 technical replicates in each tissue and detectable in both tissues, the number of genes are shown in the parentheses). A robust linear regression fitting and the corresponding R2 value are presented in each plot.
Wang et al. BMC Genomics 2006 7:59 doi:10.1186/1471-2164-7-59