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Open Access Research article

Detection of transcriptional difference of porcine imprinted genes using different microarray platforms

Shengdar Tsai13, Bashir Mir13, Amy C Martin13, Jose L Estrada13, Steve R Bischoff13, Wen-ping Hsieh4, Joseph P Cassady1, Bradley A Freking2, Dan J Nonneman2, Gary A Rohrer2 and Jorge A Piedrahita13*

Author Affiliations

1 Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA

2 USDA/ARS, U.S. Meat Animal Research Center, Clay Center, NE, USA

3 Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA

4 Bioinformatics Research Center, North Carolina State University, Raleigh, NC, USA

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BMC Genomics 2006, 7:328  doi:10.1186/1471-2164-7-328

Published: 28 December 2006

Additional files

Additional file 1:

Example of printing defect in glass oligonucleotide arrays. Example of printing defects associated with glass long oligonucleotide arrays juxtaposed with well defined spots from one region of the microarray. These defects are characteristic of spot overprinting and have a clear impact on array to array variability. Spots that are obviously defective were removed prior to array quantitation.

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Open Data

Additional file 2:

Primers used in qRT-PCR validation of microarray data. These are the primer sequences used in qPCR validation of the microarray data.

Format: DOC Size: 45KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data