Figure 5.

Comparison of effects of dilution of A. thaliana spike-in reference RNA in solution (target) and dilution of on-chip, spotted reference oligonucleotide (probe) in mouse cRNA hybridization reactions. Background-subtracted signal intensities are plotted against relative solution cRNA or attached oligonucleotide spot concentrations. Several murine genes with a range of expression levels were employed for slide-to-slide normalization of the spike-in RNA dilution series hybridization results.