Figure 1.

Schematic of the design of one sub-grid of the ToxArray™. Each sub-grid is printed in quadruplicate by one pin. Printing proceeds left-to-right across rows and top row to bottom row. Spot A (bottom row) represents a buffer-only negative control that is printed immediately following another buffer-only spot. B₁ through B₃ spots are 100 μM A. thaliana spots which reach saturation. Spots C₁ through C₃ are buffer-only spots that are printed immediately following B spots. The average signals from these spots were used to calculate the amount of cross-spot contamination (relative to Spot A) using the formula: