Figure 2.

Deviation in log2 intensity following TwoRA (Δlog2IN) as a function of probeset 3' distance rank. Δlog2IN values on the y-axis were calculated by subtracting the mean OneRA from the mean TwoRA probeset log2 intensities. The x-axis shows the ranks of probesets locations. Probesets locations are relative to the 3' end of the transcripts (refer to methods for more details). Since the probesets locations have a skewed distribution, their ranks were plotted instead of their absolute values; this allows dispersion of data points. The actual probesets locations that correspond to the rank intervals on the x-axis are shown on the blue horizontal axis on the top of the figure. The regression line is shown in red. Only data from the DRG preparation were used, similar results were obtained with the SN and