Table 3

Primers used for semi-nested RT-PCRs.

Primer name

Primer sequence

Target

OL1760

AACTAACGCTATATAAGTATCAGTTTCTGTACTTTATTG

L. major splice-leader sequence

OL2147

CTCCTTCCGGACAACAGTGCCGGTGC

3' of LmjF28.1480

OL2164

CGGCGGTGCTTTTGCA

3' of LmjF28.1470

OL2165

CTACAATCATGACGAT

3' of LmjF19.0120

OL2166

GATGGCAAGCATGATC

3' of LmjF29.0070

OL2167

GAATAGCATTGCAAAC

3' of LmjF32.0070

OL2168

GCTCTGATTCTCGCGA

3' of LmjF33.1340

OL2169

CGAGGGCTATCATCAA

3' of LmjF35.2720

Primers used to amplify syntaxin-specific products from L. major promastigotes cDNA.

Besteiro et al. BMC Genomics 2006 7:250   doi:10.1186/1471-2164-7-250