Table 1

Known unigenes showing significant expression differences between symbiotic and aposymbiotic anemones. Identification was based on significant blast hits (E values < 10^-4) from GenBank. Ratios of expression from all microarray analyses and selected Q-RT-PCR analyses are shown. Arrows indicate the direction of expression as a function of symbiosis: up- or down-regulation. Those genes highlighted in bold are discussed in the text. Significance is indicated as follows: P < 0.05 (), P < 0.01 (*).
Unigenes grouped based on functional category	Genbank Accession ID	OSU-CGRB ID	Expression as function of symbiosis

			Microarray	Q-RT-PCR

Cell Growth and Maintenance
von Willebrand factor	DQ309533	AeOSU006130	↑ 1.92 *	↑ 1.82 *
Tubulin alpha chain	DQ309523	AeOSU007825	↓ 1.20 *
Myosin regulatory light chain 2	DQ309534	AeOSU008456	↓ 1.37 *
Beta-tubulin	DQ309552	AeOSU009795	↓ 1.39 *
Collagen alpha-1 chain, type XII	DQ309546	AeOSU000073	↓ 1.94 **

Metabolism and Energy Pathways
Phytanoyl-CoA hydroxylase	DQ309528	AeOSU005830	↑ 1.54 *	↑1.59 *
Selenoprotein W1	DQ309535	AeOSU002895	↑ 1.42 *
Long chain acyl-CoA thioesterase	DQ309538–DQ309539	AeOSU003383	↑ 1.28 *
ATP synthase, H⁺transporting, mitochondrial F0 complex	DQ309536	AeOSU000538	↓ 1.29 *
Medium chain S-acyl fatty acid synthetase thio ester hydrolase	DQ309537	AeOSU000658	↓ 1.56 *	↓ 1.32 *
Copper/zinc superoxide dismutase (SOD)	DQ309550	AeOSU001001	↓ 1.58 *	↓ 1.89 *

Protein metabolism
60S ribosomal protein L26	DQ309540	AeOSU004867	↑ 3.43 **
Plasma glutamate carboxypeptidase	DQ309521	AeOSU001186	↑ 1.70 *

Nucleic Acid Metabolism
Activating transcription factor 4 (ATF4)	DQ309526	AeOSU001247	↑ 1.51 *	↑ 2.49 *
Histone H3.3	DQ309522	AeOSU007640	↑ 1.22 *
DEAD box polypeptide 48	DQ309551	AeOSU006260	↓ 1.30 *

Signal Transduction &cell communication
Oncoprotein-induced transcript 3	DQ309527	AeOSU009888	↑ 1.40 *
Scavenger receptor class B, type 1 (CD36)	DQ309525	AeOSU008133	↑ 1.34 *
Sphingosine 1-phosphate phosphatase 2	DQ309531–DQ309532	AeOSU005519	↓ 1.50 *	↓ 1.63 *
Betaine homocysteine methyl transferase	DQ309529	AeOSU002910	↓ 1.56 *	↓ 4.18 *
Calcium/calmodulin-dependent protein kinase IV	DQ309544	AeOSU005891	↓ 1.57 *
Prohibitin protein	DQ309542–DQ309543	AeOSU001676	↓ 2.21 *

Transport
Membrane-bound transferrin-like protein p97	DQ309541	AeOSU001619	↓ 1.29 *

Unclassified
Death-associated protein 1 (DAP-1)	DQ309530	AeOSU008955	↑ 1.39 *
Human headcase homolog (HECA)	DQ309548	AeOSU009119	↑ 1.33 *
Guanine nucleotide binding protein, gamma 5 or 12	DQ309547	AeOSU008880	↑ 1.29 *
SEC31-like 2 isoform A	DQ309524	AeOSU009211	↑ 1.17 *
Methylmalonate semialdehyde dehydrogenase	DQ309545	AeOSU005938	↓ 1.37 *

Rodriguez-Lanetty et al. BMC Genomics 2006 7:23 doi:10.1186/1471-2164-7-23