Open Access Research article

The evolving doublecortin (DCX) superfamily

Orly Reiner1*, Frédéric M Coquelle15, Bastian Peter2, Talia Levy1, Anna Kaplan1, Tamar Sapir1, Irit Orr3, Naama Barkai1, Gregor Eichele4 and Sven Bergmann12

Author Affiliations

1 Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel

2 Department of Medical Genetics, University of Lausanne, Switzerland

3 Department of Biological Services, Weizmann Institute of Science, Rehovot, Israel

4 Max-Planck Institute, Hannover, Germany

5 CNRS – UMR 6026, Université de Rennes 1, Equipe SDM, Campus de Beaulieu – Bât. 13, 35042 Rennes cedex, France

For all author emails, please log on.

BMC Genomics 2006, 7:188  doi:10.1186/1471-2164-7-188

Published: 26 July 2006

Additional files

Additional File 1:

Supplementary Fig. 1: Detailed list and sequences of all the DCX proteins used in this study. The list contains the set of proteins with two domains. In addition, the N-terminal domains including proteins with single domains more similar to the N-terminal domain of DCX, and the C-terminal domains including proteins with single domains more similar to the C-terminal domain of DCX are presented in this set.

Format: DOC Size: 722KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional File 2:

Supplementary Fig. 2: Synthenic positions of mouse and human DCX domain proteins.

Format: DOC Size: 26KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional File 3:

Supplementary Fig. 3: CLUSTAL W (1.83) multiple sequence alignment of the DCX proteins with tandem domains, N-terminal domains and C-terminal domains.

Format: DOC Size: 204KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional File 4:

Supplementary Fig. 4: ML tree of the N-terminal DCX domain proteins from different species. Bootstrap values are indicated.

Format: JPEG Size: 1.1MB Download file

Open Data

Additional File 5:

Supplementary Fig. 5: ML tree of the C-terminal DCX domain proteins from different species. Bootstrap values are indicated.

Format: JPEG Size: 796KB Download file

Open Data

Additional File 6:

Supplementary Fig. 6: Western blot analysis using anti-FLJ46154 antibodies. The specificity of the antibodies was verified using cells transfected with Flag-FLJ46154 (A) compared with the preimmune serum. (B) Anti-FLJ46154 antibodies recognize a protein of approximate size 78 kDa in mouse brain extract.

Format: JPEG Size: 69KB Download file

Open Data

Additional File 7:

Supplementary Fig. 7: Proposed nomenclature for the doublecortin superfamily.

Format: DOC Size: 19KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data