Table 5

Comparison of amino acid usage between two clusters of probable membrane associated proteins and between potential highly and lowly expressed genes

N. equitans


Amino acids

Upper cluster genes

Lower cluster genes

Highly expressed genes

Lowly expressed genes



Number

(%)

Number

(%)

Number

(%)

Number

(%)


Phe

771

7.88*

649

4.20

367

3.55

402

5.20

Leu

1708

17.46*

1394

9.01

954

9.24

912

11.81

Ser

626

6.40

1060

6.85

383

3.71

409

5.30

Tyr

585

5.98

1117

7.22+

429

4.15

494

6.40

Cys

33

0.34

424

2.74+

46

0.45

85

1.10

Trp

102

1.04

137

0.89

95

0.92

77

1.00

Pro

329

3.36

597

3.86

488

4.72

314

4.07

His

96

0.98

118

0.76

155

1.50

61

0.79

Gln

155

1.58

347

2.24+

243

2.35

137

1.77

Arg

178

1.82

345

2.23

444

4.30

204

2.64

Ile

1373

14.04*

1604

10.37

866

8.38

940

12.17

Met

190

1.94*

168

1.09

226

2.19

128

1.66

Thr

426

4.35

879

5.68+

484

4.69

311

4.03

Asn

405

4.14

1384

8.95+

446

4.32

459

5.94

Lys

627

6.41

1504

9.72+

1099

10.64

840

10.88

Val

571

5.84

864

5.59

836

8.09

389

5.04

Ala

558

5.70*

600

3.88

703

6.81

344

4.45

Asp

267

2.73

724

4.68+

516

5.00

327

4.23

Glu

341

3.49

816

5.28+

895

8.66

508

6.58

Gly

441

4.51

737

4.76

655

6.34

383

4.96


Values marked with *or + are significantly (p < 0.001) more frequent in upper or lower cluster gene products respectively and the values in bold faces indicate significant (p < 0.001) overrepresentation of corresponding amino acids in potential highly expressed genes.

Das et al. BMC Genomics 2006 7:186   doi:10.1186/1471-2164-7-186

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