Email updates

Keep up to date with the latest news and content from BMC Genomics and BioMed Central.

Open Access Methodology article

Predicting candidate genes for human deafness disorders: a bioinformatics approach

Rami Alsaber, Christopher J Tabone and Raj P Kandpal*

Author Affiliations

Department of Biological Sciences, Fordham University Bronx, NY 10458, USA

For all author emails, please log on.

BMC Genomics 2006, 7:180  doi:10.1186/1471-2164-7-180


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2164/7/180


Received:20 March 2006
Accepted:19 July 2006
Published:19 July 2006

© 2006 Alsaber et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

There are more than 50 genes for autosomal dominant and autosomal recessive nonsyndromic hereditary deafness that are yet to be cloned. The human genome sequence and expression profiles of transcripts in the inner ear have aided positional cloning approaches. The knowledge of protein interactions offers additional advantages in selecting candidate genes within a mapped region.

Results

We have employed a bioinformatic approach to assemble the genes encoded by genomic regions that harbor various deafness loci. The genes were then in silico analyzed for their candidacy by expression pattern and ability to interact with other proteins. Such analyses have narrowed a list of 2400 genes from suspected regions of the genome to a manageable number of about 140 for further analysis.

Conclusion

We have established a list of strong candidate genes encoded by the regions linked to various nonsyndromic hereditary hearing loss phenotypes by using a novel bioinformatic approach. The candidates presented here provide a starting point for mutational analysis in well-characterized families along with genetic linkage to refine the loci. The advantages and shortcomings of this bioinformatic approach are discussed.

Background

Hearing loss, acquired or genetic, is a major worldwide public health concern. Numerous genes have been linked to hearing disorders [1]. These disorders may be syndromic or nonsyndromic; conductive, sensorineural, or mixed; and prelingual or postlingual [2]. The various genetic forms of hearing loss are distinguished based on otologic, audiologic and physical examination combined with linkage analysis. Some representative deafness genes that have been identified include the Alport syndrome (COL4A3, COL4A4 or COL4A5 genes), branchio-oto-renal syndrome (EYA1 gene), Mohr-Tranebjaerg syndrome (TIMM8A gene), Pendred syndrome (SLC26A4 gene), Jervell and Lange-Nielsen Syndrome (KVLQT1 and KCNE1 genes), Usher syndrome with its several types, Norrie disease (NDP gene), DFNB1 (GJB2 gene), DFN3 (POU3F4 gene), DFNB4 (SLC26A4 gene), DFNA6/14 (WFS1 gene), and several others [3,4]. The mutational analysis of genes such as GJB2 (encoding the protein connexin 26) and GJB6 (encoding the protein connexin 30) [3,5,6] has aided diagnosis and geneticcounselling.

Syndromic hearing loss is associated with a variety of other clinical findings and is relatively less prevalent. In contrast, nonsyndromic hearing loss accounts for more than 70% of deafness cases and involves autosomal as well as X or Y -linked deafness phenotypes [7]. The molecular causes of nearly all nonsyndromic hearing loss are associated with inner ear structural damage, and changes in both the inner and the middle ear [8]. Mutations in genes such as the ACTG1, COCH, COL11A2, DFNA5, EYA4, GJB2, GJB6, KCNQ4, MYO6, MYO7A, TECTA, TMC1, and WFS1, as well as altered expression of genes such as GJB3 and MYO1A have been associated with the autosomal dominant types that are generally progressive and involve changes in inner ear [9-11]. The autosomal recessive phenotypes are associated with mutations in genes such as the CDH23, CLDN14, ESPN, GJB2, GJB6, MYO15A, MYO6, MYO7A, OTOF, PCDH15, SLC26A4, STRC, TECTA, TMC1, TMIE, TMPRSS3, and USH1C, as well as altered expression of GJB3 [8].

The map locations of a large number of nonsyndromic autosomal recessive deafness phenotypes are known, but the specific genes responsible for all these phenotypes have not been identified [4]. The cloning of genes involved in such phenotypes requires refinement of the suspected genomic interval to as short a region as possible by linkage analysis. However, it is not always possible to map a gene within an interval that is amenable for mutation analysis. The mutation analysis of all genes encoded by a large genomic interval is extremely labor-intensive. We describe here a bioinformatic approach that can reduce the candidate genes to a manageable number for mutation analysis. Initially, all the genes from a particular locus are cross-referenced to the databases of expressed mouse inner ear genes and the expressed human cochlear genes. The alternative procedure included a search for interacting proteins for the gene products mapping to the candidate region. As presented here, this approach has led to a set of specific candidate genes.

Results and discussion

The locations of 23 autosomal dominant and 27 autosomal recessive nonsyndromic deafness phenotypes mapped to several chromosomes downloaded from hereditary hearing loss homepage are shown in Tables 1 and 2[4]. Additional loci for nonsyndromic conditions are mapped to chromosomes 1, 8, X and Y [4]. The hereditary hearing loss homepage is updated on a regular basis. The marker boundaries of these locations encompass between 1.4 and 18.6 million basepairs (Mbp) for various loci. To generate a set of candidate genes for the listed loci, a strategy schematically represented in Figure 1 was followed. The determination of coding sequences and/or genes in a genomic region was made by Unigene [12]. However, the genes encoded in a large genomic interval are too many to be characterized by mutational analysis in a gene-by-gene approach. Therefore, we used the human cochlea and mouse inner ear expression databases [13,14] to eliminate from the candidate list certain genes that were not expressed in these organs. Such in silico expression analysis relies on the assumption that the expression databases are comprehensive. However, the characterization of all transcripts expressed in the ear is far from complete. We, therefore, introduced another step in our candidate gene strategy by taking advantage of the human protein reference database (HPRD) and generated a list of interacting genes for every gene mapping to candidate deafness loci [15]. The rationale for protein interaction is as follows. If a gene encoded in the candidate region interacts with a gene that is either involved in inner ear development/function, or a protein shows interaction with more than one candidate genes mapping to different loci, then such a gene is likely to be involved in the phenotype in question. The interaction pattern of the gene products from Usher syndrome is a good example to illustrate this point. The known gene products for several Usher syndrome loci are known to form interactions in vivo [16]. The mutation of each one of these genes affects protein interactions and influences Usher type 1 phenotype [17]. The five forms of Usher syndrome have defects in myosin VIIA, harmonin, cadherin 23, protocadherin 15, and a putative scaffolding protein sans. Harmonin binds sans, and it also binds myosin VIIA and protocadherin 15 [34]. The role of cadherins in mediating cell-cell interaction is well-characterized. Furthermore, interactions of harmonin (USH1C) with USH2A, USH2C and USH2B are mediated by PDZ domains [35,36]. In retrospect, if the interacting protein strategy had been used to select candidate genes for Usher syndrome subtypes, it is likely that several genes could have been eliminated from consideration. Therefore, it is reasonable to assume that physical interactions will exist between proteins that are involved in inner ear developmental pathway or inner ear signal transduction pathways, and mutations in any one protein of the pathway is likely to give the same altered phenotype. If proteins of interacting networks can be identified or predicted, then such genes are natural candidates for a given phenotype. The above hypothesis is the underlying rationale for incorporating interacting proteins as a criterion for selecting candidate genes presented in this paper. Briefly, the strategy is as follows. First, assemble the genes encoded in all candidate intervals, list the proteins that interact with genes in the candidate region, and then search for candidates on different loci that interact with a common protein. Such a criterion will fulfil the rationale of putative involvement of proteins at two different loci involved in a common biological process, and by association the respective genes mapping to two different loci will be considered as candidates.

Table 1. Autosomal dominant nonsyndromic loci.

Table 2. Autosomal recessive nonsyndromic loci.

thumbnailFigure 1. Schematic flow for information processing to predict candidate genes. The rectangles contain tasks that were processed in the sequence as indicated by arrows.

The application of candidate gene isolation is demonstrated for the autosomal dominant condition DFNA27. The gene is mapped to the genomic interval 4q12 spanning 15 Mbp [4]. This region codes for 36 known and 30 hypothetical proteins (Table 3) [18]. The comparison of these genes to expression databases reduced the list to 10 genes from the human cochlear database and three found in the mouse inner ear (Table 4) [13,14,19]. The possibility remained for the elimination of a stronger candidate just on the basis that it did not score a hit in expression databases. To avoid such an error, we have assembled lists of interacting proteins by using the human protein reference database (HPRD) [15] for every gene identified by GeneRetriever® from the candidate region. If an interactor of a gene in the candidate interval is expressed in inner ear then the gene is considered a candidate. Alternatively, the interacting genes from a specific locus list were compared against lists from other loci to identify if a hit was scored against proteins among two or more lists. The original genes corresponding to such interactor(s) were considered as candidates for the respective deafness loci. The strong candidates, based on the above analyses, for various deafness loci are presented in Table 5.

Table 3. GeneRetriever list of known genes found within the DFNA27 locus.

Table 4. Cochlear-expressed EST found within DFNA27 locus.

Table 5. List of candidates for various deafness loci.

In principle, the interactions-based strategy can be targeted to identify candidates for deafness if a database for interacting proteins involved in inner ear development and function is available. For example, oncomodulin and prestin are expressed in outer hair cells [20]. The protein interaction approach could link the possible candidate genes to specific cochlear cells by identifying known interactants. If the interactors happen to map to a region harboring a deafness gene, such interactors are obvious candidates for mutational analysis. However, such an approach will require identification of interacting proteins. The primary limitation of the in silico approach described here is inadequate description of interacting protein networks.

The strong candidate list includes genes such as various cadherins, collagens, some cytoskeletal components and a number of growth factors and inner ear specific transcripts. For example, HAT (Human airway trypsin-like protease) from the DFNA27 locus is known to enhance cell growth and IL-8 production. It has been implicated in induction of PAR-2 (protease activated receptor)-mediated IL-8 release in psoriasis vulgaris [21]. Because HAT is expressed in the ear, and protease activated receptor (PAR-2) has the ability to activate G-proteins followed by an increase in calcium ion concentration, we consider HAT as a candidate. KDR(kinase insert domain receptor), a vascular endothelial growth factor(VEGF) receptor-type 2, from the same locus shows age-dependent expression in the inner ear [22]. Our analyses indicated that only a fraction (200/2400) of genes mapping to various genomic intervals was expressed in the inner ear. We attribute these observations to depth of inner ear libraries. It is likely that the genes being scored in these libraries have multiplicity for certain transcripts and absence of other transcripts. For example, out of 153 genes at the DFNA7 locus, only 18 genes are present in the cochlear library. We cannot reasonably rule out the expression of the remaining 135 genes in the inner ear. Therefore, the approach presented here will be more comprehensive if we do not include ear expression in this scheme. Consequently, in a second attempt to mine the protein-interaction data obtained from the HRPD, we analyzed all genes encoded in the candidate intervals for their interactors. The interaction data were considerably exhaustive and resulted in many more possible candidates with their expression not reported in the ear expression library. A summary of gene numbers at different loci before and after interacting proteins analysis using the ear-expression scheme is presented in Table 6. The mouse syntenic genes are also indicated in these results. The number of unfiltered candidate genes for each locus obtained by interacting proteins analysis is shown in Table 7. To elucidate the relevance of genes not found in the ear-expression library as possible candidates, we performed a literature search cross-referencing the identified gene with any reported hearing-associated condition in humans or other model animals. Some of these genes were linked to ear-development or hearing impairment as a secondary or unrelated symptom of other conditions. For example, Neurod1 gene mapping to DFNB27 locus was not reported in any of the inner ear libraries. However, it appears to participate in the development of the auditory system as NeuroD1 null mice exhibited severe reduction of sensory neurons in the cochlear-vestibular ganglion [23]. E2F3, a transcription factor of the E2F family mapping to the DFNA21 locus, may be indirectly implicated by its ability to regulate cell proliferation possibly during the developmental stages [24]. Other candidate genes from the unfiltered candidate analysis for the various loci are listed in Table 8. Thus the unfiltered strategy adds 51 candidates for 25 loci and expands the candidate list to 92 genes for further mutation analysis.

Our approach indicated the presence of possible candidates within most of the mapped loci. However, prediction of candidate genes was not easy for loci indicated by asterisks in Table 7, because the genes mapping to these loci did not fulfil the criteria we have employed. We further examined these genes on the basis of their reported function. The following description pertains to specific genes that are not indicated in the candidate lists. Within the DFNA16 locus, SCNA3 and SCNA2, both being voltage-gated sodium channels, can be considered candidates based on involvement of related sodium channels in hearing [25]. Similarly, ATP2C1 in DFNA18 locus is a likely candidate because mutations in a related ATPase have been shown in mice that are profoundly deaf and have a balance defect [26]. The EphB1 gene, within the DFNA18 locus, plays a major role during the development of the inner ear in mice [27]. The DFNA23 locus has six1 gene that plays a pivotal role in the control of the mouse otic vesicle patterning [28]. Neugrin, mapping to DFNA30 locus, appeared to be an appropriate candidate as it was shown to be up-regulated throughout neuronal differentiation [29]. A possible candidate for the DFNA47 locus is the transcription factor Nfib, an essential player in the maturation of lungs and brain development [30]. The splicing regulation carried out by Pnn, mapping to the DFNB5 region, is a reasonable candidate [31]. We believe the genes presented in this article may serve as starting candidates toward identifying molecular mechanisms for specific deafness phenotypes.

Table 6. Summary of gene numbers from expression-library filtered analysis.

Table 7. Summary of gene numbers from unfiltered analysis.

Table 8. Candidate genes from unfiltered HPRD analysis*.

Conclusion

We have used an in silico strategy to assemble a list of candidate genes that are positionally linked to and could be causing specific nonsyndromic hereditary hearing loss conditions. As presented here, a list of 2378 genes mapping to various genomic intervals have been narrowed down to 92 genes as candidates. These candidates may be analyzed for mutations in various deafness phenotypes in parallel with attempts to further narrow down the suspected region by genetic linkage analysis. It warrants mention that the potential of the approach presented here will be better harnessed as more information becomes available about inner ear transcripts and protein interaction networks.

Methods

Generating list of loci for in silico prediction

The list of most current information and identified loci for the various nonsyndromic hearing loss and syndromic forms was obtained from the Hereditary Hearing Loss Homepage and the survey of latest literature [4]. The list of deafness loci with unknown specific genes for the autosomal dominant, autosomal recessive, and syndromic forms was also compiled from the same web based source.

GeneRetriever for EST identification within each deafness locus

A list of all cloned and identified genes from within each of the listed genomic intervals was obtained using GeneRetriever®, a Perl-based data mining software that has a simple graphical user interfaces [12]. It automatically retrieves from either NCBI or Ensembl databases information that includes all genes and transcripts located in a genomic interval flanked by two genetic markers.

Database analysis

The list of genes and transcripts for each specific locus obtained using GeneRetriever® was compared against two sets of ear gene-expression databases. The first set includes genes expressed in the developing ear [13]. This list is a compilation of the numerous genes that are expressed at different stages during inner ear development in two animal species. The second set was obtained from fetal cochlear cDNA library and EST database (updated as of 2002) of the Morton Hearing Research Group [14]. The data present in this set was adapted from Unigene [12]. The database has 14,805 ESTs, and 12,624 ESTs are sorted by Unigene into 4,519 independent clusters. Unigene did not classify the remaining ESTs due to factors such as possible contaminating sequences, very small inserts, or excessive repetitive elements. For a gene within a particular locus to be considered for candidacy, it has to be present in either of the above two databases. Genes that were not present in either expression databases were initially eliminated from consideration. It warrants mention that functional significance of expressed sequences in human and mouse inner ear has been used to propose deafness candidates [32,33].

Human reference protein database

In comparing the two sets of databases to the list of genes and transcripts within each hereditary hearing loss locus obtained using GeneRetriever®, we were able to compile a list of possible candidate genes for the various loci. To further narrow-down and refine this list, we obtained a list of all known interacting genes for each of the known and candidate genes using the Human Reference Protein Database (HRPD)[15]. The interacting proteins for all the genes within the mapped loci were obtained regardless of whether the gene is present in the two data sets of inner-ear expressed transcripts. In our first attempt of mining the data, if a gene is not present in the data set but its interacting proteins are expressed or present in the cochlea or identified in the table of gene expression in the developing ear, then this gene is considered a candidate. In our second attempt, we removed the ear-expression filter requirement. Therefore, any interacting and repeating protein was given consideration. Identifying candidate interacting genes that repeat in many loci supported their candidacy, resulting in a more comprehensive candidate list.

Authors' contributions

RPK was responsible for conceiving this project, designing the strategy, preparation of the manuscript and overall supervision. RA wrote some basic programs for comparisons, carried out data base mining, assembled the set of candidate genes and participated in manuscript preparation. CJT was involved in the initial stages of the project. All authors read and approved the final manuscript.

Acknowledgements

This work was supported in part by Faculty Research Award and Ames Award to RPK.

References

  1. Gorlin RJ, Toriello HV, Cohen MM, eds: Hereditary Hearing Loss and its Syndromes. Oxford University Press, NY; 1995. OpenURL

  2. Van Camp G, Willems PJ, Smith RJ: Nonsyndromic hearing impairment: unparalleled heterogeneity.

    Am J Hum Genet 1997, 60:758-64. PubMed Abstract OpenURL

  3. Petit C: From deafness genes to hearing mechanisms: harmony and counterpoint.

    Trends Mol Med 2006, 12:57-64. PubMed Abstract | Publisher Full Text OpenURL

  4. Van Camp G, Smith RJH: The Hereditary Hearing Loss Homepage. [http://dnalab-www.uia.ac.be/dnalab/hhh] webcite

    2005.

  5. Scott DA, Kraft ML, Carmi R, Ramesh A, Elbedour K, Yairi Y, Srisailapathy CR, Rosengren SS, Markham AF, Mueller RF, Lench NJ, Van Camp G, Smith RJ, Sheffield VC: Identification of mutations in the connexin 26 gene that cause autosomalrecessive nonsyndromic hearing loss.

    Hum Mutat 1998, 11:387-94. PubMed Abstract | Publisher Full Text OpenURL

  6. Rabionet R, Lopez-Bigas N, Arbones ML, Estivill X: Connexin mutations in hearing loss, dermatological and neurological disorders.

    Trends Mol Med 2002, 8:205-12. PubMed Abstract | Publisher Full Text OpenURL

  7. Friedman TB, Griffith AJ: Human nonsyndromic sensorineural deafness.

    Annu Rev Genomics Hum Genet 2003, 4:341-402. PubMed Abstract | Publisher Full Text OpenURL

  8. Van Laer L, Cryns K, Smith RJ, Van Camp G: Nonsyndromic hearing loss.

    Ear Hear 2003, 24:275-88. PubMed Abstract | Publisher Full Text OpenURL

  9. Alexandrino F, Oliveira CA, Reis FC, Maciel-Guerra AT, Sartorato EL: Screening for mutations in the GJB3 gene in Brazilian patients with nonsyndromic deafness.

    J Appl Genet 2004, 45:249-54. PubMed Abstract | Publisher Full Text OpenURL

  10. Donaudy F, Ferrara A, Esposito L, Hertzano R, Ben-David O, Bell RE, Melchionda S, Zelante L, Avraham KB, Gasparini P: Multiple mutations of MYO1A, a cochlear-expressed gene, in sensorineural hearing loss.

    Am J Hum Genet 2003, 72:1571-7. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  11. Petersen MB: Non-syndromic autosomal-dominant deafness.

    Clin Genet 2002, 62:1-13. PubMed Abstract | Publisher Full Text OpenURL

  12. Pontius JU, Wagner L, Schuler GD: UniGene: a unified view of the transcriptome.

    The NCBI Handbook. Bethesda (MD): National Center for Biotechnology Information 2003. OpenURL

  13. Holme RH, Bussoli TJ, Steel KP: Table of gene expression in the developing ear. [http://www.ihr.mrc.ac.uk/Hereditary/genetable] webcite

    2003.

  14. Skvorak AB, Morton CC: The Human Cochlear EST Database. [http://hearing.bwh.harvard.edu/estinfo.HTM] webcite

    2002.

  15. Peri S, Navarro JD, Amanchy R, Kristiansen TZ, Jonnalagadda CK, Surendranath V, Niranjan V, Muthusamy B, Gandhi TK, Gronborg M, Ibarrola N, Deshpande N, Shanker K, Shivashankar HN, Rashmi BP, Ramya MA, Zhao Z, Chandrika KN, Padma N, Harsha HC, Yatish AJ, Kavitha MP, Menezes M, Choudhury DR, Suresh S, Ghosh N, Saravana R, Chandran S, Krishna S, Joy M, Anand SK, Madavan V, Joseph A, Wong GW, Schiemann WP, Constantinescu SN, Huang L, Khosravi-Far R, Steen H, Tewari M, Ghaffari S, Blobe GC, Dang CV, Garcia JG, Pevsner J, Jensen ON, Roepstorff P, Deshpande KS, Chinnaiyan AM, Hamosh A, Chakravarti A, Pandey A: Development of human protein reference database as an initial platform for approaching systems biology in humans. [http://www.hprd.org/] webcite

    Genome Res 2003, 13:2363-2371. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  16. Adato A, Michel V, Kikkawa Y, Reiners J, Alagramam KN, Weil D, Yonekawa H, Wolfrum U, El-Amraoui A, Petit C: Interactions in the network of Usher syndrome type 1 proteins.

    Hum Mol Genet 2005, 14:347-56. PubMed Abstract | Publisher Full Text OpenURL

  17. Kimberling WJ, Moller C: Clinical and molecular genetics of Usher syndrome.

    J Am Acad Audiol 1995, 6:63-72. PubMed Abstract OpenURL

  18. Clément-Ziza M, Brody Y, Munnich A, Lyonnet S, Besmond C: GeneRetriever: software to extract all genes and transcripts in between two genetic markers to assist design of human custom microarrays.

    BioTechniques 2005, 39:180-184. PubMed Abstract OpenURL

  19. Robertson NG, Khetarpal U, Gutierrez-Espeleta GA, Bieber FR, Morton CC: Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening.

    Genomics 1994, 23:42-50. PubMed Abstract | Publisher Full Text OpenURL

  20. Sakaguchi N, Henzel MT, Thalmann I, Thalmann R, SCHULTE BA: Oncomodulin is expressed exclusively by outer hair cells in the organ of Corti.

    J Histochem Cytochem 1998, 46:29-40. PubMed Abstract | Publisher Full Text OpenURL

  21. Iwakiri K, Ghazizadeh M, Jin E, Fujiwara M, Takemura T, Takezaki S, Kawana S, Yasuoka S, Kawanami O: Human airway trypsin-like protease induces PAR-2-mediated IL-8 release in psoriasis vulgaris.

    J Invest Dermatol 2004, 122:937-44. PubMed Abstract | Publisher Full Text OpenURL

  22. Picciotti P, Torsello A, Wolf FI, Paludetti G, Gaetani E, Pola R: Age-dependent modifications of expression level of VEGF and its receptors in the inner ear.

    Exp Gerontol 2004, 39:1253-8. PubMed Abstract | Publisher Full Text OpenURL

  23. Liu M, Pereira FA, Price SD, Chu MJ, Shope C, Himes D, Eatock RA, Brownell WE, Lysakowski A, Tsai MJ: Essential role of BETA2/NeuroD1 in development of the vestibular and auditory systems.

    Genes Dev 2000, 14:2839-2854. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  24. Black EP, Hallstrom T, Dressman HK, West M, Nevins JR: Distinctions in the specificity of E2F function revealed by gene expression signatures.

    Proc Natl Acad Sci U S A 2005, 102:15948-53. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  25. Koay G, Heffner R, Heffner H: Behavioral audiograms of homozygous med(J) mutant mice with sodium channel deficiency and unaffected controls.

    Hearing Res 2002, 171:111-118. Publisher Full Text OpenURL

  26. Shull GE, Okunade G, Liu LH, Kozel P, Periasamy M, Lorenz JN, Prasad V: Physiological functions of plasma membrane and intracellular Ca2+ pumps revealed by analysis of null mutants.

    Ann N Y Acad Sci 2003, 986:453-60. PubMed Abstract | Publisher Full Text OpenURL

  27. Pickles JO, Claxton C, Van Heumen WR: Complementary and layered expression of Ephs and ephrins in developing mouse inner ear.

    J Comp Neurol 2002, 449:207-16. PubMed Abstract | Publisher Full Text OpenURL

  28. Ozaki H, Nakamura K, Funahashi J, Ikeda K, Yamada G, Tokano H, Okamura HO, Kitamura K, Muto S, Kotaki H, Sudo K, Horai R, Iwakura Y, Kawakami K: Six1 controls patterning of the mouse otic vesicle.

    Development 2004, 131:551-62. PubMed Abstract | Publisher Full Text OpenURL

  29. Ishigaki S, Niwa J, Yoshihara T, Mitsuma N, Doyu M, Sobue G: Two novel genes, human neugrin and mouse m-neugrin, are upregulated with neuronal differentiation in neuroblastoma cells.

    Biochem Biophys Res Commun 2000, 279:526-33. PubMed Abstract | Publisher Full Text OpenURL

  30. Steele-Perkins G, Plachez C, Butz KG, Yang G, Bachurski CJ, Kinsman SL, Litwack ED, Richards LJ, Gronostajski RM: The transcription factor gene Nfib is essential for both lung maturation and brain development.

    Mol Cell Biol 2005, 25:685-98. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  31. Chiu Y, Ouyang P: Loss of Pnn expression attenuates expression levels of SR family splicing factors and modulates alternative pre-mRNA splicing in vivo.

    Biochem Biophys Res Commun 2006, 341:663-71. PubMed Abstract | Publisher Full Text OpenURL

  32. Skvorak AB, Weng Z, Yee AJ, Robertson NG, Morton CC: Human cochlear expressed sequence tags provide insight into cochlear gene expression and identify candidate genes for deafness.

    Hum Mol Genet 1999, 8:439-52. PubMed Abstract | Publisher Full Text OpenURL

  33. Klockars T, Perheentupa T, Dahl HH: In silico analyses of mouse inner-ear transcripts.

    J Assoc Res Otolaryngol 2003, 4:24-40. PubMed Abstract | Publisher Full Text OpenURL

  34. Kalay E, de Brouwer AP, Caylan R, Nabuurs SB, Wollnik B, Karaguzel A, Heister JG, Erdol H, Cremers FP, Cremers CW, Brunner HG, Kremer H: A novel D458V mutation in the SANS PDZ binding motif causes atypical Usher syndrome.

    J Mol Med 2005, 83:1025-32. PubMed Abstract | Publisher Full Text OpenURL

  35. Reiners J, van Wijk E, Märker T, Zimmermann U, Jürgens K, te Brinke H, Overlack N, Roepman R, Knipper M, Kremer H, Wolfrum U: Scaffold protein harmonin (USH1C) provides molecular links between Usher syndrome type 1 and type 2.

    Hum Mol Genet 2005, 14:3933-43. PubMed Abstract | Publisher Full Text OpenURL

  36. van Wijk E, van der Zwaag B, Peters T, Zimmermann U, Te Brinke H, Kersten FF, Märker T, Aller E, Hoefsloot LH, Cremers CW, Cremers FP, Wolfrum U, Knipper M, Roepman R, Kremer H: The DFNB31 gene product whirlin connects to the Usher protein network in the cochlea and retina by direct association with USH2A and VLGR1.

    Hum Mol Genet 2006, 15:751-65. PubMed Abstract | Publisher Full Text OpenURL