Open Access Highly Accessed Research article

The genome of the square archaeon Haloquadratum walsbyi : life at the limits of water activity

Henk Bolhuis1*, Peter Palm2, Andy Wende2, Michaela Falb2, Markus Rampp3, Francisco Rodriguez-Valera4, Friedhelm Pfeiffer2 and Dieter Oesterhelt2

Author Affiliations

1 Department of Microbial Ecology, Centre for Ecological and Evolutionary Studies, University of Groningen, The Netherlands

2 Department of Membrane Biochemistry, Max Planck Institute of Biochemistry, Martinsried, Germany

3 Computer Center of the Max-Planck-Society, Max Planck Institute of Plasma Physics, Garching, Germany

4 Evolutionary Genomics Group and División de Microbiología, Universidad Miguel Hernandez, Alicante, Spain

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BMC Genomics 2006, 7:169  doi:10.1186/1471-2164-7-169

Published: 4 July 2006

Abstract

Background

The square halophilic archaeon Haloquadratum walsbyi dominates NaCl-saturated and MgCl2 enriched aquatic ecosystems, which imposes a serious desiccation stress, caused by the extremely low water activity. The genome sequence was analyzed and physiological and physical experiments were carried out in order to reveal how H. walsbyi has specialized into its narrow and hostile ecological niche and found ways to cope with the desiccation stress.

Results

A rich repertoire of proteins involved in phosphate metabolism, phototrophic growth and extracellular protective polymers, including the largest archaeal protein (9159 amino acids), a homolog to eukaryotic mucins, are amongst the most outstanding features. A relatively low GC content (47.9%), 15–20% less than in other halophilic archaea, and one of the lowest coding densities (76.5%) known for prokaryotes might be an indication for the specialization in its unique environment

Conclusion

Although no direct genetic indication was found that can explain how this peculiar organism retains its square shape, the genome revealed several unique adaptive traits that allow this organism to thrive in its specific and extreme niche.