|
Hybridization signal strength of five genes found to be expressed using the array tube but not using the Affymetrix chip, respectively, compared with the results of quantitative PCR experiments (non-stimulated Coga cells). Array tube: relative transmission values, normalized overall. Affymetrix chip: relative fluorescence value, normalized overall |
||||
| Gene |
Affymetrix chip |
Array tube |
PCR |
Quantitative RT-PCR |
|
|
||||
| PAI-1 |
not present |
0,53 |
4,34*10-7 ng |
|
| MMP7 |
not present |
0,52 |
1,02*10-6 ng |
|
| MMP8 |
not present |
0,47 |
positive |
|
| CST5 |
not present |
0,89 |
positive |
|
| CTSK |
273,8+) |
0,43 |
8,13*10-6 ng |
|
|
+) this value is below the cut off of 300. | ||||
Schüler et al. BMC Genomics 2006 7:144 doi:10.1186/1471-2164-7-144 |
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