Table 1

List of the Chlamydiales-specific proteins

Protein Loci
Protein (Name/Size)
C. tra
C. mur
C. pne
C. cav
C. abo
Protochlam.
Next best BLAST Hit (E value, protein length)

CT007 (AAC67597)
hypoth, 316aa
0
8e-164
8e-108
6e-108
3e-105
8e-10, 320aa
Plasmodium yoelii (0.063, 1491aa)
CT011 (AAC67601)
hypoth, 418aa
0
0
7e-151
2e-155
1e-157
7e-47, 445aa
Desulfotalea psychrophila (1e-06, 337aa)
CT016 (AAC67606)
hypoth, 242aa
2e-139
3e-119
1e-82
4e-89
3e-86
2e-37, 233aa
Rickettsia sibirica (0.20, 1851aa)
CT017 (AAC67607)
hypoth. 433aa
0
0
1e-122
7e-133
9e-135
4e-52, 431aa
Thermo. tengcongensis (1e-06, 306 aa)
CT021 (AAC67611)
hypoth, 247aa
2e-139
4e-107
1e-47
3e-56
4e-53
2e-13, 245aa
Xenopus laevis (1.7, 600aa)
CT031 (AAC67621)
hypoth, 100aa
4e-51
4e-42
2e-25
4e-26
2e-26
0.010, 107aa
Mus musculus (3.6, 384aa)
CT038 (AAC67628)
hypoth, 116aa
1e-63
2e-49
4e-25
1e-25
2e-23
2e-09, 109aa
Neurospora crassa (2.8, 1223aa)
CT053 (AAC67644)
hypoth, 148aa
2e-61
4e-57
2e-51
1e-50
1e-48
5e-29, 155aa
Xenopus tropicalis (4.7, 1049aa)
CT066 (AAC67657)
hypoth, 158aa
3e-77
1e-75
8e-65
3e-65
5e-65
6e-41, 165aa
Vibrio parahaemolyticus (1.8, 92aa)
CT079 (AAC67670)
hypoth, 147aa
4e-81
1e-71
2e-06
1e-29
1e-29
3e-06, 172aa
Leuconostoc mesenteroides (0.11, 263aa)
CT131 (AAC67722)
PTMP, 1138aa
0
0
0
0
0
3e-33, 1348aa
Plasmodium yoelii (0.31, 592aa)
CT181 (AAC67772)
hypoth, 236aa
7e-131
1e-113
3e-77
7e-81
4e-80
2e-10, 200aa
Acinetobacter sp. (0.94, 371aa)
CT260 (AAC67853)
hypoth, 163aa
9e-91
9e-81
5e-62
3e-68
1e-67
4e-22, 159aa
Sinorhizobium meliloti (0.18, 166aa)
CT266 (AAC67859)
hypoth, 393aa
0
5e-179
9e-103
5e-120
2e-119
4e-08, 498aa
Xanthomonas axonopodis (2.8, 189aa)
CT271 (AAC67864)
hypoth, 95aa
1e-39
2e-36
7e-25
1e-25
9e-26
1e-10, 97aa
Gibberella zeae (3.6, 1362)
CT273 (AAC67866)
hypoth, 188aa
2e-89
1e-77
2e-60
5e-63
2e-63
0.001, 149aa
Corynebacterium efficiens (0.34, 640aa)
CT274 (AAC67867)
type III secretion chaperone, 139aa
3e-74
3e-70
1e-61
1e-62
1e-61
3e-31, 131aa
Synechococcus elongatus (2e-05, 363aa)
CT303 (AAC67896)
hypoth, 171aa
2e-81
5e-66
2e-40
2e-47
6e-47
3e-04, 177aa
Yersinia pestis (1.3, 373aa)
CT309 (AAC67902)
hypoth, 266aa
2e-144
2e-129
8e-86
1e-93
5e-94
2e-29, 263aa
Methanococcus jannaschii (2.6, 434aa)
CT312 (AAC67905)
pred. ferredoxin, 123aa
6e-67
6e-54
1e-30
7e-36
1e-35
1e-04, 130aa
Pan troglodytes (1.2, 1524aa)
CT350 (AAC67945)
hypoth, 566aa
0
0
2e-141
3e-157
1e-157
2e-30, 561aa
Nostoc sp. PCC 7120 (7e-06, 1547aa)
CT351 (AAC67946)
hypoth, 697aa
0
0
0
0
0
5e-53, 720aa
Rattus norvegicus (3.4, 389aa)
CT355 (AAC67950)
hypoth, 353aa
0
3e-161
3e-101
8e-101
1e-102
1e-21, 375aa
Chromobacterium violaceum (0.82, 556aa)
CT384 (AAC67980)
hypoth, 539aa
0
0
9e-106
4e-113
1e-111
2e-14, 580aa
Pseudomonas fluorescens (0.29, 314aa)
CT387 (AAC67984)
hypoth, 691aa
0
0
0
0
0
4e-160, 740aa
Rubrobacter xylanophilus (5e-10, 263aa)
CT421.2 (AAC68031)
hypoth, 53aa
5e-23
2e-22
5e-19
7e-20
4e-19
2e-12, 52aa
no other BLAST hits
CT425 (AAC68022)
hypoth, 621aa
0
0
0
0
0
0, 665aa
Bordetella parapertussis (4e-10, 767aa)
CT429 (AAC68026)
hypoth, 329aa
4e-165
4e-162
5e-149
2e-151
7e-151
8e-96, 388aa
Geobacter sulfurreducens (2e-04, 146aa)
CT443 (AAC68042)
OmcB, 553aa
0
0
0
0
0
4e-66, 558aa
Rhodopirellula baltica (3e-25, 907aa)
CT446 (AAC68045)
EUO protein, 183aa
1e-78
8e-72
1e-50
2e-53
1e-52
4e-41, 139aa
Magnaporthe grisea (2.1, 1259aa)
CT474 (AAC68074)
LysM protein, 336aa
2e-168
9e-116
5e-59
9e-71
7e-71
3e-04, 482aa
no other BLAST hits
CT504 (AAC68105)
hypoth, 288aa
2e-127
1e-105
4e-64
2e-69
2e-68
1e-23, 376aa
Mus musculus (3.0, 961aa)
CT538 (AAC68140)
hypoth, 238aa
8e-124
1e-120
5e-105
1e-108
1e-108
9e-65, 244aa
Fritschea bemisiae (3e-59, 241aa) Enterococcus faecalis (0.067, 541aa)
CT546 (AAC68148)
predicted OMP, 289aa
3e-163
4e-157
6e-87
1e-92
1e-93
3e-23, 249aa
Legionella pneumophila (0.042, 254aa)
CT547 (AAC68149)
hypoth, 318aa
2e-175
7e-158
8e-90
1e-92
2e-93
8e-29, 468aa
Caenorhabditis elegans (4e-04, 1168aa)
CT548 (AAC68150)
hypoth, 194aa
3e-89
2e-67
1e-48
2e-49
3e-48
0.004, 185aa
Drosophila melanogaster (0.17, 826aa)
CT556 (AAC68158)
hypoth, 159aa
1e-92
1e-82
9e-59
1e-60
1e-59
2e-06, 157aa
Xenopus laevis (1.8, 1636aa)
CT560 (AAC68162)
hypoth, 278aa
1e-135
2e-125
1e-86
4e-94
5e-93
0.002, 281aa
no other BLAST hits
CT573 (AAC68175)
hypoth, 409aa
0
1e-179
9e-90
3e-113
2e-106
1e-43, 472aa
Pseudomonas syringae (0.46, 9376aa)
CT583 (AAC68185)
Virulence plasmid pGP6 related protein, 263aa
5e-134
6e-118
4e-82
4e-91
2e-92
1e-46, 245aa
Crocosphaera watsonii (0.036, 333aa)
CT590 (AAC68192)
hypoth, 954aa
0
0
0
0
0
8e-96, 989aa
Porphyromonas gingivalis (0.003, 645aa)
CT602 (AAC68205)
hypoth, 130aa
5e-60
1e-55
2e-35
3e-37
1e-37
2e-19, 131aa
Actinobacillus pleuropneumoniae (2.8, 300aa)
CT616 (AAC68220)
hypoth, 429aa
0
0
2e-101
8e-115
3e-114
2e-09, 435aa
Giardia lamblia (0.003, 1024aa)
CT623 (AAC68227)
76 kDa Homolog, 446aa
0
2e-155
5e-152
1e-154
1e-157
1e-28, 470aa
Mycobacterium tuberculosis (0.18, 550aa)
CT631 (AAC68235)
hypoth, 84aa
1e-32
2e-31
1e-24
3e-28
8e-28
1e-11, 82aa
No other BLAST hits
CT635 (AAC68239)
hypoth, 144aa
2e-62
5e-58
6e-45
1e-50
2e-51
4e-04, 146aa
Gallus gallus (0.003, 1748aa)
CT642 (AAC68246)
hypoth, 271aa
2e-134
2e-116
2e-74
2e-79
8e-81
2e-22, 300aa
Rattus norvegicus (1.6, 509aa)
CT652.1 (AAC68830)
hypoth, 59aa
5e-26
3e-24
7e-12
3e-12
7e-13
3e-05, 75aa
Parachlamydia sp. (0.12, 70aa)
CT666 (AAC68261)
hypoth, 83aa
5e-40
9e-31
3e-35
1e-36
9e-37
0.43, 92aa
Sacchromyces cerevisiae (3.7, 604aa)
CT667 (AAC68262)
hypoth, 149aa
3e-78
1e-65
3e-51
9e-55
4e-55
8e-16, 154aa
Legionella pneumophila (0.005, 577aa)
CT670 (AAC68265)
hypoth, 168aa
1e-29
1e-27
2e-23
4e-24
3e-23
9e-10, 165aa
No other BLAST hits
CT734 (AAC68329)
hypoth, 221aa
7e-106
1e-95
2e-51
1e-56
2e-57
1e-08, 240aa
Caenorhabditis briggsae (1.1, 1072aa)
CT764 (AAC68359)
hypoth, 268aa
2e-154
4e-113
1e-46
7e-59
4e-59
2e-05, 256aa
Tetraodon nigroviridis (1.5, 388aa)
CT788 (AAC68383)
leader (60) peptide-periplasmic, 166aa
1e-79
3e-61
9e-25
4e-34
1e-32
0.003, 158aa
Caenorhabditis elegans (0.19, 2271aa)
CT809 (AAC68405)
hypoth, 103aa
1e-55
2e-44, 146aa
5e-17
2e-23
2e-21
4e-05, 158aa
Canis familiaris (2.1, 1831aa)
CT838 (AAC68435)
YjgP/YjgQ family protein, 366aa
0
0
e-126
e-130
3e-128
6e-24, 360aa
Prochlorococcus marinus (0.18, 592aa)
CT845 (AAC68442)
hypoth, 92aa
2e-46
7e-33
1e-19
2e-17
1e-17
8e-10, 85aa
Candida albicans (2.7, 557aa)
CT849.1 (AAC68455)
hypoth, 62aa
2e-28
9e-24
5e-10
2e-09
1e-08
1e-05, 81aa
No other BLAST hits
CT865 (AAC68463)
hypoth, 329aa
2e-180
8e-146
7e-76
1e-77
4e-78
9e-23, 352aa
Bacteroides thetaiotaomicron (0.20, 808aa)

The Chlamydiales-specific proteins were identified by systematic BLASTP searches using Chl. trachomatis ORFs as probes, where the only significant BLAST hits obtained were for chlamydiae species (see Methods section). Accession numbers of the query sequences are shown beside the Chl. trachomatis loci (CT number) in the first column. The observed expected (E) values for various chlamydiae homologs as well as the first non-chlamydial hit in the BLAST results are given. E values of 0.0 indicate an extremely high degree of similarity between proteins sequences. The second column describes the function and the size of each query protein (protein sizes are described for other chlamydiae only if they noticeably differ from the query protein). Abbreviations are: C. tra, Chl. trachomatis; C. mur; Chl. muridarum; C. pne, Chlam. pneumoniae; C. cav, Chlam. caviae; C. abo, Chlam. abortus; hypoth, hypothetical protein; Omc; Outer membrane cytochrome; OMP, outer membrane protein; Protochlam, Protochlamydia; Pred., Predicted; PTMP, Possible transmembrane protein.

Griffiths et al. BMC Genomics 2006 7:14   doi:10.1186/1471-2164-7-14