Table 1

Significant up- and down-regulated genes from SAM analysis of microarray experiments on 3 sets of monozygotic twins discordant for autism diagnosis with log2(ratio) ≥ ± 0.58

Genbank#

Gene name or description

Mean log2(ratio)*

qPCR¥


Upregulated (log2(ratio) ≥ 0.58)

R45254

Unknown protein

1.19

AA448599

F13A1, clotting factor XIIIa precursor

1.08

1.05

AA676466

ASS, argininosuccinate synthetase (aa 1–412)

0.92

AA992985

Unknown protein

0.88

AA676405

ASS, argininosuccinate synthetase (aa 1–412)

0.85

1.47

W07099

NAGLU, N-acetylglucosaminidase, alpha

0.85

-0.28

AA044267

P2X5a

0.80

T49652

FLAP, ALOX5AP

0.79

1.01

H57830

histone H1(0) (aa 1–194)

0.78

R00276

CD38 alt

0.76

AA488070

Unknown protein

0.75

W69399

histone H1(0) (aa 1–194)

0.74

H09567

PAG1

0.74

AA609189

Unknown protein

0.73

H02307

FLAP, ALOX5AP

0.70

N50114

PAG1

0.69

AI091671

Unknown protein

0.67

N70181

PLEKHG1

0.66

H24011

Homeodomain-like protein

0.64

AA412520

Unknown protein

0.63

AA521362

CR2 receptor

0.62

AI371096

DAPK1, death-associated protein kinase 1

0.62

0.65

T61343

IL6ST, IL6 signal tranducer, gp130

0.59

0.58

N29918

ZBTB10

0.59

T90067

EIF2C2

0.59

0.99

Downregulated (log2(ratio) ≤ -0.58)

T67053

IGLC2

-2.39

W73790

IGLL1

-2.00

H18423

Unknown protein

-1.98

AA448157

CYP1B1

-0.95

AA644099

Unknown protein

-0.89

AA933744

ECAT11

-0.84

AI018127

Unknown protein

-0.83

AA451886

CYP1B1

-0.77

AA682565

Unknown protein from neuroblastoma

-0.72

AI223429

Unknown protein

-0.69

AA450353

ELMOD1

-0.69

AA873578

IGHG1

-0.67

R33402

SAMSN1

-0.67

-0.61

AA173755

ROBO1, roundabout 1

-0.66

-0.93

AA022886

retinal degeneration B beta

-0.64

AA063573

SAMSN1

-0.64

H99699

mitochondrial aconitase

-0.63

AI290663

CYBASC3

-0.60

AA449333

Rab22b

-0.58


*Mean log2(ratio) of gene expression in lymphoblastoid cell lines from children exhibiting classic autism to cell lines from less affected monozygotic twin sibs. SAM analysis revealed 1200 significant genes with an FDR of 26.4%. Only genes for which microarray data is available for all 3 sets of twins are included in this table.

¥Mean log2(ratio) of qPCR data from 3 sets of monozygotic twins. Gene expression was analyzed in triplicate assays (or duplicate for F13A1, EIF2C2, and SAMSN1) and the mean log2(ratio) for each respective gene was averaged among the 3 sets of twins. Only one gene, NAGLU, out of the 9 tested from this table was not confirmed by qPCR, possibly because of suboptimal choice of primers for qPCR.

Genes in boldface type have been shown to be relevant to neurological development, structure, or function (See Table 3).

Hu et al. BMC Genomics 2006 7:118   doi:10.1186/1471-2164-7-118

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