Research article
Bacterial genome adaptation to niches: Divergence of the potential virulence genes in three Burkholderia species of different survival strategies
-
* Corresponding authors: William C Nierman wnerman@tigr.org - David DeShazer david.deshazer@us.army.mil
1 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
2 Department of microbiology and plant pathology, University of Georgia, Athens, GA 30602, USA
3 US Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, MD, 21702 USA
BMC Genomics 2005, 6:174 doi:10.1186/1471-2164-6-174
Published: 7 December 2005Additional files
Additional File 1:
Global relatedness of the genomes of Bm, Bp, and Bt. Comparative genomics display tool ACT (Wellcome Trust Sanger Institute) was used to depict the syntenic fragments present between Bp and Bm and Bp and Bt based on BLASTN data.
Format: EPS Size: 3.7MB Download file
Additional File 2:
RT-PCR confirmation of microarray data. Microarray and RT-PCR data of brain infusion vs. mouse spleen (see Fig. 1A) is compared using the thirteen randomly picked genes. For each gene, primer sequences, expected amplicon size, and annotation are shown.
Format: EPS Size: 1.3MB Download file
Additional File 3:
Genes significantly up- or down expressed in mouse spleen and liver compared to cultures as shown in Fig. 1A.
Format: PDF Size: 104KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional File 4:
Comparison of the whole genomes in Fig. 3A.
Format: PDF Size: 299KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional File 5:
Genes up- or down-expressed in vivo in Fig. 3B.
Format: PDF Size: 79KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional File 6:
The 30 in vivo-expressed genes that are degenerated or unique to Bm in Fig. 3B.
Format: PDF Size: 47KB Download file
This file can be viewed with: Adobe Acrobat Reader