|
Expected red:green ratios in two-color hybridizations |
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| Array |
Pool in Green Channel |
Pool in Red Channel |
Group A Ratio |
Group B Ratio |
Group C Ratio |
Group D Ratio |
|
|
||||||
| 1 |
P7-D1 |
P10-D1 |
0.008 |
5 |
5 |
5 |
| 2 |
P8-D1 |
P9-D1 |
0.2 |
0.2 |
125 |
0.2 |
| 3 |
P9-D1 |
P8-D1 |
5 |
5 |
0.008 |
5 |
| 4 |
P10-D1 |
P7-D1 |
125 |
0.2 |
0.2 |
0.2 |
| 5 |
P7-D2 |
P8-D2 |
0.2 |
125 |
0.2 |
0.2 |
| 6 |
P8-D2 |
P7-D2 |
5 |
0.008 |
5 |
5 |
| 7 |
P9-D2 |
P10-D2 |
0.2 |
0.2 |
0.2 |
125 |
| 8 |
P10-D2 |
P9-D2 |
5 |
5 |
5 |
0.008 |
| 9 |
P7-D3 |
P9-D3 |
0.04 |
25 |
25 |
0.04 |
| 10 |
P8-D3 |
P10-D3 |
0.04 |
0.04 |
25 |
25 |
| 11 |
P9-D3 |
P7-D3 |
25 |
0.04 |
0.04 |
25 |
| 12 |
P10-D3 |
P8-D3 |
25 |
25 |
0.04 |
0.04 |
| 13* |
P7-D4 |
P7-D4 |
1 |
1 |
1 |
1 |
| 14* |
P8-D4 |
P8-D4 |
1 |
1 |
1 |
1 |
| 15* |
P9-D4 |
P9-D4 |
1 |
1 |
1 |
1 |
| 16* |
P10-D4 |
P10-D4 |
1 |
1 |
1 |
1 |
|
*Self-to-self hybridizations Pools in each channel are labeled based on their pool and dilution numbers in Table 6. Groups are sets of transcripts at the same concentration as defined in Table 4. | ||||||
External RNA Controls Consortium BMC Genomics 2005 6:150 doi:10.1186/1471-2164-6-150 |
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