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Concentration of controls in dilution 1 pools for modified latin square experiments |
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| Pool |
Concentration Group A (Controls 1–24) |
Concentration Group B (Clones 25–48) |
Concentration Group C (Clones 49–72) |
Concentration Group D (Clones 73–96) |
|
|
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| Pool 7 |
125 |
1 |
5 |
25 |
| Pool 8 |
25 |
125 |
1 |
5 |
| Pool 9 |
5 |
25 |
125 |
1 |
| Pool 10 |
1 |
5 |
25 |
125 |
|
Concentration is given as mass ratios, so that "125" represents 1:125,000 or 1 ng of RNA transcript per 125 ng of background RNA where the spike amount is adjusted for its length. | ||||
External RNA Controls Consortium BMC Genomics 2005 6:150 doi:10.1186/1471-2164-6-150 |
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