Table 3

Annotation and Gene Ontology characteristics of 11 honey bee EST contigs sharing significant similarity with mammalian but not with other vertebrate or invertebrate sequences. In all cases, the best match was with human proteins. For these 11 out of 23 contigs we could retrieve functional information.

Apis EST contig

E-score valuea

GO, Biological processb

Human LOCUS ID GenBank annotation

Additional information

Human

Insect


437

2e-67

NA

without result

NM_019116: ubiquitin binding protein

ubiquitin-specific protease domain

663

3e-10

NA

without result

NM_182830: MAM domain

contactin 5; neural adhesion molecule

1081

5e-07

9.7

without result

NM_013041: RAB3A interacting protein (rabin3)-like1

guanin nucleotide exchange factor domain

1425

8e-27

NA

without result

NM_182565: hypothetical protein MGC29814

TBP-associated factor 4; TATA box binding protein

1674

4e-82

NA

without result

NM_172374: interleukin 4 induced 1

none

1953

7e-11

NA

without result

NM_024707: gem (nuclear organelle) associated protein

spliceosomal snRNP biogenesis

2807

8e-07

5e-04

regulation of physiological process

NM_138457: forkhead box P4

transcription factor activity

2896

4e-05

0.002

reproduction, metabolism

NM_004654: ubiquitin-specific protease 9

ubiquitin thiolesterase activity

3167

2e-27

7e-05

cell communication

NM_033046: rhotekin

signal transduction

3347

5e-26

7.5

without result

NM_014006: PI-3-kinase-related kinase SMG-1

involved in nonsense-mediated mRNA decay

3374

1e-10

0.18

cell communication,

NM_014035: sorting nexing 24

intracellular signaling cascade


aE-value of the contig alignment with human or known insect sequences, NA: did not show any alignment; bGene Ontology results on Biological Process, FatiGO level 3, cadditional information obtained from Entrez Gene – NCBI and GOA link (GOAnnotations@EBI – European Bioinformatics Institute).

Nunes et al. BMC Genomics 2004 5:84   doi:10.1186/1471-2164-5-84

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