Percentage of honey bee and Drosophila ESTs representing putative species-specific genes (blue bars) in relation to ESTs that represent genes solely shared within the insect clade (pink bars), or that have higher similarity with eukaryotes other than the insect clade (yellow bars). In separate comparisons, the Apis mellifera contigs (ORESTES + NCBI dbESTs, n = 5,000), AmORESTES (n = 5,000), AmNCBI dbESTs (n = 5,000), and Drosophila melanogaster ORESTES contigs (n = 5,000) were analyzed against protein databases of an insect (Anopheles gambiae) and several non-insect species (C. elegans, protozoans, fungi and H. sapiens) with completely sequenced genomes. The cut-off E-value in these comparisons was set at 10-6.
Nunes et al. BMC Genomics 2004 5:84 doi:10.1186/1471-2164-5-84