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Open Access Research article

In silico identification and expression of SLC30 family genes: An expressed sequence tag data mining strategy for the characterization of zinc transporters' tissue expression

Michel Seve*, Fabrice Chimienti, Séverine Devergnas and Alain Favier

Author Affiliations

DRFMC/SCIB/LAN, CEA/Grenoble, Grenoble, France

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BMC Genomics 2004, 5:32  doi:10.1186/1471-2164-5-32

Published: 23 May 2004

Additional files

Additional File 1:

Expression of ZnT-5 and ZnT-7 mRNA in human tissues. ZnT-5 and ZnT7 mRNA expressions were assessed by PCR using cDNA libraries prepared from different human tissues: Brain, heart, kidney, spleen, liver, plasma blood leukocyte, lung, muscle, intestine, ovary, prostate, testis. Specifically amplified products were visualized by agarose gel after ethidium bromide staining. β-actin specific primers were also used as a control to ensure equal template concentrations.

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Additional File 2:

Expression of ZnT-8 mRNA in human tissues. ZnT-7 mRNA expression was assessed by PCR using cDNA libraries prepared from different human tissues: 1: Brain, 2: Heart, 3: Kidney, 4: Spleen, 5: Liver, 6: Colon, 7: Lung, 8: Small intestine, 9: Muscle, 10: Stomach, 11: Testis, 12: Placenta, 13: Salivary, 14: Thyroid, 15: Adrenal, 16: Pancreas, 17: Ovary, 18: Uterus, 19: Prostate, 20: Skin, 21: Plasma blood leukocyte, 22: Bone marrow, 23: Fetal brain, 24: Fetal liver. Specifically amplified products were visualized by agarose gel after ethidium bromide staining. β-actin specific primers were also used as a control to ensure equal template concentrations.

Format: PPT Size: 89KB Download file

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Open Data