Table 1

Amplification factors

tot RNA starting amount [ng]
Repetitions
Range of amplified RNA [μg]
Average amount of amplified RNA [μg]
Range of amplification factors
Average amplification factor*

100–200
14
1–4.4
2.49
200–560
368
1,000–3,200
16
12–36
22
150–500
253

* calculated under the assumption that 5% of total RNA corresponds to poly (A)+ RNA

Schneider et al. BMC Genomics 2004 5:29   doi:10.1186/1471-2164-5-29