Table 1

Amplification factors

tot RNA starting amount [ng]

Repetitions

Range of amplified RNA [μg]

Average amount of amplified RNA [μg]

Range of amplification factors

Average amplification factor*


100–200

14

1–4.4

2.49

200–560

368

1,000–3,200

16

12–36

22

150–500

253


* calculated under the assumption that 5% of total RNA corresponds to poly (A)+ RNA

Schneider et al. BMC Genomics 2004 5:29   doi:10.1186/1471-2164-5-29

Open Data