Research article

Relationship between gene co-expression and probe localization on microarray slides

Yuval Kluger^{* 1,2} , Haiyuan Yu^{* 1} , Jiang Qian^{* 1,3} and Mark Gerstein¹

¹Department of Molecular Biophysics and Biochemistry, Yale University, PO Box 208114, New Haven, CT 06520, USA

²Current address: Cell Biology, New York University School of Medicine, New York, NY 10016, USA

³Current address: Wilmer Institute, Johns Hopkins School of Medicine, Baltimore, MD, 21287, USA

author email corresponding author email* Contributed equally

BMC Genomics 2003, 4:49doi:10.1186/1471-2164-4-49

Published:	10 December 2003

Abstract

Background

Microarray technology allows simultaneous measurement of thousands of genes in a single experiment. This is a potentially useful tool for evaluating co-expression of genes and extraction of useful functional and chromosomal structural information about genes.

Results

In this work we studied the association between the co-expression of genes, their location on the chromosome and their location on the microarray slides by analyzing a number of eukaryotic expression datasets, derived from the S. cerevisiae, C. elegans, and D. melanogaster. We find that in several different yeast microarray experiments the distribution of the number of gene pairs with correlated expression profiles as a function of chromosomal spacing is peaked at short separations and has two superimposed periodicities. The longer periodicity has a spacing of 22 genes (~42 Kb), and the shorter periodicity is 2 genes (~4 Kb).

Conclusion

The relative positioning of DNA probes on microarray slides and source plates introduces subtle but significant correlations between pairs of genes. Careful consideration of this spatial artifact is important for analysis of microarray expression data. It is particularly relevant to recent microarray analyses that suggest that co-expressed genes cluster along chromosomes or are spaced by multiples of a fixed number of genes along the chromosome.