Table 1

Mass yields generated by IVT from varying amounts of restricted DNA.

Input (ng)

Yield (ug)

n-fold amp*


50.0

78.7

1574

25.0

69.8

2790

10.0

54.2

5420

5.0

25.8

5163

2.5

10.3

4114

50.0 PCR

15

300


*Yield and fold amplification may be overestimated for low starting concentration due to non-specific or template independent products (see text). The yield for the PCR amplification could be improved by introducing more cycles. However, this would likely increase bias.

Liu et al. BMC Genomics 2003 4:19   doi:10.1186/1471-2164-4-19

Open Data