Table 9

The effect of the amount of template BC2 total RNA on the fidelity, reproducibility and yield of amplification.a

Input total RNA

3 μg

1 μg

300 ng

100 ng

30 ng

10 ng


T7 primer used (μg)

0.5

0.2

0.1

0.1

0.1

0.1

Average correlation coefficient

Amplified vs. gold standard (fidelity)

0.80 ± 0.04 (n = 4)

0.81 ± 0.05 (n = 3)

0.84 ± 0.05 (n = 2)

ND

ND

ND

Amplified vs. amplified (reproducibility)

0.92 ± 0.04 (n = 4)

0.88 ± 0.04 (n = 3)

0.90 (n = 2)

ND

ND

ND

Yield (μg)

15.2 ± 5.6 (n = 4)

7.4 ± 4.6 (n = 3)

3.1 ± 1.6 (n = 2)

0.60 ± 0.3 (n = 2)

0.33 ± 0.2 (n = 3)

0.11 ± 0.1 (n = 3)

Fold of amplification

507 ± 186

740 ± 460

1033 ± 530

600 ± 300

1100 ± 667

1100 ± 1000


aData were obtained from comparing expression levels of 13,164 clones using the described selection criteria. ND = not determined due to insufficient yield of aRNA for microarray hybridization after single round of amplification.

Zhao et al. BMC Genomics 2002 3:31   doi:10.1186/1471-2164-3-31

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