Table 8

Evaluation of the reproducibility of T7 based amplification.a,b

Protocol

Affymetrixc

Affymetrix w/o ligase

Jeffrey lab with G-50

Jeffrey lab

poly(A)+ RNA


Average correlation coefficient

Same day

0.91 ± 0.04 (n = 3)

0.98 (n = 2)

0.95 ± 0.01 (n = 3)

0.97 (n = 2)

0.97 ± 0.01 (n = 3)

Different day

0.84 ± 0.05 (n = 3)

0.88 ± 0.05 (n = 3)

0.88 ± 0.03 (n = 4)

0.90 ± 0.03 (n = 3)

0.89 ± 0.03 (n = 2)

Overall

0.86 ± 0.05 (n = 6)

0.89 ± 0.05 (n = 5)

0.89 ± 0.03 (n = 7)

0.91 ± 0.03 (n = 5)

0.92 ± 0.05 (n = 5)


aData were obtained from comparing expression level of 11,123 clones using the described selection criteria. bAmplification was done using 3 μg BC2 total RNA. cNote that hybridizations were performed on Stanford printed cDNA microarrays, not Affymetrix arrays.

Zhao et al. BMC Genomics 2002 3:31   doi:10.1186/1471-2164-3-31

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