|
Efficacy of amplification using 3 μg total RNA from BC91 and different ds cDNA cleanup methods, with or without TS primer. |
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| Column used for ds cDNA cleanup |
Bio-6 |
G-50 |
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|
|
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| Amplification Protocol |
TS |
w/o TS |
TS |
w/o TS |
|
|
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| Yield of aRNA (μg) |
7.6 ± 1.0 (n = 5) |
11.1 ± 2.2 (n = 3) |
8.9 ± 3.8 (n = 6) |
10.0 ± 5.1 (n = 6) |
| Fold of amplification |
253 ± 33 |
370 ± 73 |
297 ± 126 |
333 ± 170 |
Zhao et al. BMC Genomics 2002 3:31 doi:10.1186/1471-2164-3-31 |
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