Figure 3.

Effect of 3' arm length and ligation temperature on allele discrimination. The 3' arm of the matching OCPs formed 12, 15, 20, or 23 basepairs, as indicated, when annealed to the oligonucleotide target. Ligation reaction temperatures were as indicated.

Faruqi et al. BMC Genomics 2001 2:4   doi:10.1186/1471-2164-2-4
Download authors' original image