Figure 2.

Kinetics of the ligation reaction. All reactions contained an oligonucleotide target with the wild type T at the SNP position. Reactions labeled ''Match'' had the corresponding 3' A on the OCP (a). Reactions labeled ''Mismatch'' had T on the OCP 3' end (b). Reactions contained 20 unit Ampligase/reaction (closed circles), 40 unit Ampligase/reaction (open circles), or 80 unit Ampligase/reaction (triangles). OCP was labeled on the 5' end with γ-32P-dATP and T4 polynucleotide kinase. Ligation was quantified as percent conversion to circular form on a 6% DNA sequencing gel.

Faruqi et al. BMC Genomics 2001 2:4   doi:10.1186/1471-2164-2-4
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