Comparison of three next-generation sequencing platforms for metagenomic sequencing and identification of pathogens in blood
1 Naval Medical Research Center, NMRC-Frederick, 8400 Research Plaza, Fort Detrick, Frederick, MD 21702, USA
2 Henry M. Jackson Foundation, 6720-A Rockledge Drive, Suite 100, Bethesda, MD 20817, USA
3 Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD 20814, USA
BMC Genomics 2014, 15:96 doi:10.1186/1471-2164-15-96Published: 4 February 2014
Additional file 1: Figure S1:
Read mapping against segment 5 of Influenza genome. Reads resulting from Ion Torrent 314 chip were mapped to the reference Influenza a H1NI segment 5 [NCBI accession: NC_002019], using CLC Genomics Workbench version 6.0 at default parameters. Coordinates of reference genome segment are displayed along the top and G/C content is graphed below reference in pink.
Format: PNG Size: 93KB Download file
Additional file 2: Table S1:
Mapped reads by Influenza A segment for MiSeq and PGM replicatesa. a: Statistics for one of two independent libraries at low stringency parameters.
Format: DOCX Size: 46KB Download file
Additional file 3: Table S2:
Proportion of mapped reads as a function of Influenza A genome segment size for MiSeq and PGM replicatesa. a: Statistics for one of two independent libraries at low stringency parameters.
Format: DOCX Size: 15KB Download file
Additional file 4: Figure S2:
Predicted free energy of individual Influenza genome segments. Using CLC Genomics Workbench version 6.0, Gibb's free energy was predicted for each genome segment of the NCBI reference strain Influenza A virus (A/Puerto Rico/8/34(H1N1)).
Format: PNG Size: 648KB Download file
Additional file 5: Table S3:
Sequences of primers used in this study.
Format: DOCX Size: 78KB Download file