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Open Access Research article

A genome-wide association analysis for porcine serum lipid traits reveals the existence of age-specific genetic determinants

Arianna Manunza1, Joaquim Casellas2, Raquel Quintanilla3, Rayner González-Prendes1, Ramona N Pena4, Joan Tibau5, Anna Mercadé2, Anna Castelló12, Nitdia Aznárez3, Jules Hernández-Sánchez3 and Marcel Amills12*

Author Affiliations

1 Department of Animal Genetics, Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Universitat Autònoma de Barcelona, Bellaterra 08193, Spain

2 Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain

3 IRTA, Genètica i Millora Animal, Lleida 25198, Spain

4 Departament de Producció Animal, Agrotecnio Center, Universitat de Lleida, Lleida 25198, Spain

5 IRTA, Finca Camps i Armet, 17121 Monells, Spain

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BMC Genomics 2014, 15:758  doi:10.1186/1471-2164-15-758

Published: 4 September 2014

Abstract

Background

The genetic determinism of blood lipid concentrations, the main risk factor for atherosclerosis, is practically unknown in species other than human and mouse. Even in model organisms, little is known about how the genetic determinants of lipid traits are modulated by age-specific factors. To gain new insights into this issue, we have carried out a genome-wide association study (GWAS) for cholesterol (CHOL), triglyceride (TRIG) and low (LDL) and high (HDL) density lipoprotein concentrations measured in Duroc pigs at two time points (45 and 190 days).

Results

Analysis of data with mixed-model methods (EMMAX, GEMMA, GenABEL) and PLINK showed a low positional concordance between trait-associated regions (TARs) for serum lipids at 45 and 190 days. Besides, the proportion of phenotypic variance explained by SNPs at these two time points was also substantially different. The four analyses consistently detected two regions on SSC3 (124 Mb, CHOL and LDL at 190 days) and SSC6 (135 Mb, CHOL and TRIG at 190 days) with highly significant effects on the porcine blood lipid profile. Moreover, we have found that SNP variation within SSC3, SSC6, SSC10, SSC13 and SSC16 TARs is associated with the expression of several genes mapping to other chromosomes and related to lipid metabolism.

Conclusions

Our data demonstrate that the effects of genomic determinants influencing lipid concentrations in pigs, as well as the amount of phenotypic variance they explain, are influenced by age-related factors.

Keywords:
Animal model; Lipids and lipoproteins; Genome-wide analysis; Gene expression