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Open Access Research article

Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431

Yanfang Yang, Hainan Zhao, Roberto A Barrero, Baohong Zhang, Guiling Sun, Iain W Wilson, Fuliang Xie, Kevin D Walker, Joshua W Parks, Robert Bruce, Guangwu Guo, Li Chen, Yong Zhang, Xin Huang, Qi Tang, Hongwei Liu, Matthew I Bellgard, Deyou Qiu*, Jinsheng Lai* and Angela Hoffman*

BMC Genomics 2014, 15:69  doi:10.1186/1471-2164-15-69

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Correction for paclitaxel extraction

Deyou Qiu   (2014-03-24 14:03)  The Research Institute of Forestry,Chinese Academy of Forestry

Culture broth was extracted with dichloromethane and mycelia was freeze-dried, pulverized and extracted with dichloromethane. Solvent was removed by reduced pressure at 36°C and the extracts were pooled. To the final crude extract, 0.344 g, 50 mL of water was added, and the mixture separated on C-18 cartridge (Fisher) with vacuum. Methanol was used to rinse the C-18 cartridge and elute the solution that contains paclitaxel. The methanol solution was dried (0.256 g) and dissolved in methanol or acetonitrile to 200 μg/μL after which it was filtered through a 0.45 nm filter.

Competing interests

None declared


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