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Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing

Vinzenz Lange1, Irina Böhme1*, Jan Hofmann2, Kathrin Lang1, Jürgen Sauter2, Bianca Schöne1, Patrick Paul1, Viviane Albrecht1, Johanna M Andreas1, Daniel M Baier2, Jochen Nething3, Ulf Ehninger23, Carmen Schwarzelt1, Julia Pingel2, Gerhard Ehninger4 and Alexander H Schmidt12

Author Affiliations

1 DKMS Life Science Lab, Fiedlerstrasse 34, 01307 Dresden, Germany

2 DKMS German Bone Marrow Center, Kressbach 1, 72072 Tübingen, Germany

3 molpe Softwareentwicklungs GmbH, 72138 Kirchentellinsfurt, Germany

4 Medizinische Klinik und Poliklinik I, Universitätsklinikum Carl Gustav Carus der Technischen Universität Dresden, Fetscherstrasse 74, 01307 Dresden, Germany

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BMC Genomics 2014, 15:63  doi:10.1186/1471-2164-15-63

Published: 24 January 2014



A close match of the HLA alleles between donor and recipient is an important prerequisite for successful unrelated hematopoietic stem cell transplantation. To increase the chances of finding an unrelated donor, registries recruit many hundred thousands of volunteers each year. Many registries with limited resources have had to find a trade-off between cost and resolution and extent of typing for newly recruited donors in the past. Therefore, we have taken advantage of recent improvements in NGS to develop a workflow for low-cost, high-resolution HLA typing.


We have established a straightforward three-step workflow for high-throughput HLA typing: Exons 2 and 3 of HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 are amplified by PCR on Fluidigm Access Array microfluidic chips. Illumina sequencing adapters and sample specific tags are directly incorporated during PCR. Upon pooling and cleanup, 384 samples are sequenced in a single Illumina MiSeq run. We developed “neXtype” for streamlined data analysis and HLA allele assignment. The workflow was validated with 1140 samples typed at 6 loci. All neXtype results were concordant with the Sanger sequences, demonstrating error-free typing of more than 6000 HLA loci. Current capacity in routine operation is 12,000 samples per week.


The workflow presented proved to be a cost-efficient alternative to Sanger sequencing for high-throughput HLA typing. Despite the focus on cost efficiency, resolution exceeds the current standards of Sanger typing for donor registration.

Human leukocyte antigen; HLA typing; NGS; Dual indexing; 4-primer approach; Amplicon-based sequencing; Fluidigm Access Array; Illumina MiSeq