Sensitivity of EXPRSS in differential expression detection for specific responses to treatment. (A-C) Venn diagram representing overlap of differential expression for flg22 treatment from four different experiments (Microarray on seedling, leaf, leaf disc and EXPRSS on leaf disc) (A) Up-regulated genes between the four methods are compared. (B) Down-regulated genes between the four methods are compared. (C) Depiction of the overlap among the sections of Venn diagram. Number under each experiment represents number of genes differentially expressed. (Numbers in black - genes spotted on ATH1 array and numbers in red- genes not spotted on ATH1 array) (D-E) Scatter plots showing fold change distribution for commonly detected genes between two experiments. (D) Comparison of log2 fold changes between leaf and seedling microarray shows more restricted dynamics on negative scale (~ − 3) than positive scale (~ + 7). (E) Similar comparison of log2 fold changes between leaf disc data from EXPRSS and NlaIII-DGE showing more even distribution (−6 to +7).
Rallapalli et al. BMC Genomics 2014 15:341 doi:10.1186/1471-2164-15-341