Table 9

Necrotrophic genes expression of putative Secreted proteins
Transcript Id Gene annotation RNAseq QPCR
30DPI 60DPI Pvalue Fold change Primers Induced 30-60 DPI Repressed 30-60 DPI
Mean* Mean* Mean St. E. Mean St. E.
e.m.s_0228_0004.4 Alpha beta hydrolase 3.2 34.5 1.4E-02 10.8
e.m.s_0245_0001.13 Alpha1,3-glucanase/mutanase 0.7 171.8 8.3E-03 245.4
e.m.s_0185_0001.10 Aryl-alcohol oxidase 0.0 21.4 5.7E-04 21.4
e.m.s_0185_0001.9 Aryl-alcohol oxidase 0.0 7.8 4.2E-02 7.8
e.m.s_0274_0001.9 Aryl-alcohol oxidase 0.0 9.6 2.1E-02 9.6
e.m.s_0491_0001.5 Aryl-alcohol oxidase 3.9 61.7 3.0E-02 15.9
e.m.s_0100_0003.8 Arylsulfatase 2.6 42.4 2.9E-02 16.4
e.m.s_0251_0003.2 Aspartic peptidase a1 11.5 46.7 3.6E-02 4
e.m.s_0056_0003.12 Aspartic-type endopeptidase 0.7 25.6 2.5E-03 36.6
e.m.s_0463_0001.8 Beta-lactamase class penicillin binding protein 6.6 324.6 1.5E-03 492.4
e.m.s_0066_0001.46 Biotrophy-associated secreted protein 2 MrBAS1 7.6 496.7 1.0E-03 66.1 MrSP2a 26.509 15.802
e.m.s_0005_0005.16 Biotrophy-associated secreted protein 2 MrBAS2 2.0 613.6 3.2E-04 322.6
e.m.s_0227_0001.20 Calcineurin-like phosphoesterase 1.8 19.0 9.5E-03 10.6
e.m.s_0066_0001.21 Carbohydrate esterase family 4 protein 0.7 83.8 1.8E-02 119.7
e.m.s_0155_0002.4 Carbohydrate esterase family 4 protein 0.0 50.5 2.1E-06 50.5
e.m.s_0008_0001.25 Carbonic anhydrase 0.7 121.3 7.0E-08 172.8
e.m.s_0190_0004.1 Carboxypeptidase cpds 3.8 460.0 4.4E-07 121.1
e.m.s_0190_0004.2 Carboxypeptidase cpds 3.1 28.5 5.1E-03 9.2
e.m.s_0176_0001.14 Cellulose-binding protein 0.0 22.2 4.7E-04 22.2
e.m.s_0058_0002.19 Cerato platanin 0.0 16.2 3.2E-03 16.2
e.m.s_0058_0002.21 Cerato-platanin 2.1 36.2 2.7E-02 18.1
e.m.s_0192_0001.2 Cerato-platanin 0.0 20.7 7.5E-04 20.7
e.m.s_0192_0001.6 Cerato-platanin 0.0 494.9 2.2E-04 494.9
e.m.s_0297_0002.3 Chitin deacetylase family 4 0.0 40.4 2.8E-02 40.5 MrCHIDACTb 5663.095 3460.583
e.m.s_0245_0001.24 Chitin synthase 0.0 2158.9 2.8E-04 2159 MrCHS 2515.177 1862.483
e.m.s_0008_0004.19 Copper amine oxidase 4.3 77.0 6.6E-03 17.9
e.m.s_0003_0001.85 Cutinase 0.0 9.5 2.3E-02 9.5
e.m.s_0017_0003.61 Cytochrome p450 0.0 32.2 9.1E-03 32.2
e.m.s_0052_0004.10 Cytochrome p450 0.7 14.9 3.2E-02 21.1
e.m.s_0470_0001.6 Cytochrome p450 monooxygenase 5.0 91.2 1.1E-04 18.3 MrCp450a 30.050 11.8905
e.m.s_0059_0001.14 Deuterolysin m35 metalloprotease 2.8 137.0 5.0E-02 48.9
e.m.s_0099_0003.13 Dioxygenase family protein 8.3 5904.3 3.6E-02 715.6
e.m.s_0061_0002.41 Exo-beta 1,3-glucanase 2.6 16.7 4.4E-02 6.4
e.m.s_0026_0005.53 Expansin family protein 0.0 1165.0 9.8E-04 1165 MrEXP-A 326.810 159.020
e.m.s_0086_0003.2 Expansin family protein 1.8 37.3 2.6E-04 20.7
e.m.s_0280_0002.5 Extracellular dioxygenase 0.0 15.1 3.4E-03 15.1
e.m.s_0107_0002.16 Extracellular triacylglycerol lipase precursor 0.0 9.8 2.0E-02 9.8
e.m.s_0107_0002.18 Extracellular triacylglycerol lipase precursor 0.0 9.8 2.3E-02 9.8
e.m.s_0352_0003.3 Extracellular triacylglycerol lipase precursor 0.0 9.7 2.1E-02 9.7
e.m.s_0054_0001.12 FAD binding domain protein 31.7 306.9 2.3E-04 9.7
e.m.s_0135_0001.27 FAD binding domain-containing protein 0.0 43.9 6.4E-06 43.9
e.m.s_0277_0002.12 FAD binding domain-containing protein 6.5 91.2 1.1E-04 14.1
e.m.s_0277_0002.13 FAD binding domain-containing protein 2.6 124.8 1.1E-04 48
e.m.s_0375_0001.9 FAD binding domain-containing protein 2.0 85.8 9.5E-06 43.1
e.m.s_0459_0001.6 FAD binding domain-containing protein 0.0 36.5 3.2E-04 36.5
e.m.s_0469_0001.4 FAD binding domain-containing protein 0.0 8.4 3.7E-02 8.4
e.m.s_0002_0010.20 F-box and fnip repeat-containing protein 114.0 3336.6 2.1E-02 29.3
e.m.s_0087_0002.1 Fruit-body specific gene a 0.7 20.9 7.7E-03 29.8
e.m.s_0053_0004.14 Fungal peroxidase 3.6 22.3 1.5E-02 6.3
e.m.s_0111_0006.5 Glucooligosaccharide oxidase 0.0 27.8 8.3E-03 27.8
e.m.s_0211_0001.14 Glucose oxidase 0.0 66.0 3.6E-07 66
e.m.s_0085_0002.37 Glycoside hydrolase family 16 protein 0.0 30.0 7.7E-05 29
e.m.s_0021_0002.67 Glycoside hydrolase family 18 protein 19.6 87.3 5.0E-02 4.5
e.m.s_0261_0004.2 Glycoside hydrolase family 3 protein 25.4 127.4 1.7E-02 5
e.m.s_0008_0004.3 Glycoside hydrolase family 30 protein 11.7 67.7 3.7E-02 6
e.m.s_0232_0001.2 Glycoside hydrolase family 35 protein 33.9 128.7 4.3E-02 3.8
e.m.s_0438_0002.3 Glycoside hydrolase family 35 protein 0.0 19.7 8.7E-04 19.7
e.m.s_0007_0001.127 Glycoside hydrolase family 5 protein 27.1 163.4 5.6E-03 6
e.m.s_0265_0002.8 Glycoside hydrolase family 5 protein 10.8 111.9 5.4E-05 10.4
e.m.s_0241_0001.11 Glycoside hydrolase family 61 protein 0.0 26.6 9.6E-03 26.6
e.m.s_0241_0001.12 Glycoside hydrolase family 61 protein 0.0 43.1 5.9E-03 43.1
e.m.s_0155_0002.8 Glycoside hydrolase family 61 protein 13.5 115.5 1.3E-02 8.6
e.m.s_0004_0003.50 Glycoside hydrolase family 9 protein 10.4 63.2 8.9E-03 6.3 MrGH9 4.311 1.827
e.m.s_0200_0003.3 Glycosyl hydrolase family 10 0.7 22.6 3.6E-02 32.2
e.m.s_0333_0001.1 Glyoxal oxidase 4.9 47.9 5.1E-03 9.8
e.m.s_0135_0001.28 gmc oxidoreductase 14.3 52.2 2.4E-02 3.6
e.m.s_0074_0003.10 Hemerythrin hhe cation binding domain-containing protein 49.9 383.1 2.1E-02 7.7
e.m.s_0039_0002.15 Hemolysin 0.7 16.1 2.3E-02 22.9
e.m.s_0062_0001.18 Heterokaryon incompatibility protein het-c 28.2 116.3 1.5E-02 4.1
e.m.s_0018_0008.40 Hydrophobin 13.2 86.6 3.6E-02 6.6
e.m.s_0018_0008.54 Hydrophobin 0.7 61.9 1.2E-05 87.1
e.m.s_0018_0008.56 Hydrophobin 0.0 256.2 4.3E-12 256 MrLM18 1573.702 1268.272
e.m.s_0058_0002.53 Hydrophobin 0.7 25.1 2.8E-03 35.7
e.m.s_0058_0002.55 Hydrophobin 9.0 304.7 5.8E-07 34.2
e.m.s_0149_0001.14 Hydrophobin 0.0 11.2 1.2E-02 11.2
e.m.s_0149_0001.16 Hydrophobin 0.0 425.5 2.3E-06 425.6 MrLM19 257.737 133.646
e.m.s_0170_0002.12 Hydrophobin 0.0 9.3 2.4E-02 9.3
e.m.s_0298_0001.2 Hydrophobin 0.0 11.4 1.2E-02 11.4
e.m.s_0149_0001.15 Hydrophobin 2 0.0 205.6 1.6E-04 205.6
e.m.s_0199_0001.3 Laccase 0.0 9.6 2.6E-02 9.6
e.m.s_0210_0002.8 Laccase 0.0 119.6 1.0E-06 119.6
e.m.s_0246_0002.7 Laccase 1.3 41.0 1.0E-02 31.8
e.m.s_0279_0002.3 Laccase 0.0 21.1 3.4E-02 21.2
e.m.s_0082_0001.16 Mannoprotein 88.8 298.1 4.7E-02 3.4
e.m.s_0030_0003.64 Metalloproteinase 18.6 306.9 8.1E-06 16.5
e.m.s_0406_0002.4 Necrosis inducing-like protein npp1 type 9.1 658.2 1.5E-04 73.1 MrNPP1 310.949 181.890
e.m.s_0051_0003.3 nhl repeat-containing protein 0.0 28.8 1.1E-04 28.8
e.m.s_0120_0002.7 Oxalate decarboxylase 9.3 57.9 4.8E-02 6.2
e.m.s_0398_0002.11 Oxidoreductase fad binding 1.8 111.0 5.4E-04 61.6
e.m.s_0639_0001.6 Para-nitrobenzyl esterase 0.0 45.8 3.2E-04 45.8
e.m.s_0082_0001.36 Peptidase m28 3.2 57.6 3.9E-02 18
e.m.s_0007_0001.138 Peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase a 0.0 12.6 3.5E-02 12.5
e.m.s_0180_0002.16 Phosphatidylserine decarboxylase 4.3 451.8 5.1E-04 105.1
e.m.s_0432_0001.5 PR-1 protein MrPR-1d 5.8 45.1 1.4E-03 7.8
e.m.s_0568_0001.7 Proline-rich antigen 7.7 1372.5 7.7E-03 177.3
e.m.s_0018_0003.1 Proline-specific peptidase 29.2 94.0 5.0E-02 3.2
e.m.s_0726_0001.2 Pyrolysin 8.1 50.9 3.5E-02 6.3
e.m.s_0125_0001.43 Riboflavin aldehyde-forming enzyme 61.2 229.9 3.8E-02 3.8
e.m.s_0040_0001.30 Ribonuclease t1 0.0 11.4 1.1E-02 11.4
e.m.s_0321_0001.8 Serine-rich protein 16.4 289.5 5.2E-04 18.1 MrSRP 14.072 5.613
e.m.s_0005_0005.47 Serine-type endopeptidase 224.0 811.1 4.1E-02 3.6 MrS-endo 3.482 0.866
e.m.s_0021_0002.36 Tripeptidyl peptidase A 0.0 20.8 6.5E-04 20.8
e.m.s_0166_0004.10 Tripeptidyl peptidase A 1.8 39.5 1.8E-04 21.9
e.m.s_0048_0003.5 Virus P4 KP4 toxin 0.0 118.9 4.5E-02 119 MrKP4 134.442 52.042
e.m.s_0014_0002.14 wsc domain-containing protein 0.0 42.6 8.7E-06 42.6
e.m.s_0010_0002.57 Zinc metalloprotease 10.7 92.8 2.6E-03 8.7

Mean* is the normalized mean based on the size factor of the libraries.

Meinhardt et al.

Meinhardt et al. BMC Genomics 2014 15:164   doi:10.1186/1471-2164-15-164

Open Data