Table 8

Biotrophic gene Expression of putative Secreted Proteins
Transcript Id Gene annotation RNAseq QPCR
30DPI 60DPI Pvalue Fold change Primers Induced 30-60 DPI Repressed 30-60 DPI
Mean* Mean* Mean St. E. Mean St. E.
e.m.s_0369_0002.2 Acetylxylan esterase 638.4 91.6 5.8E-03 7.0
e.m.s_0461_0001.1 Carbohydrate esterase family 8 protein 512.1 67.0 8.8E-04 7.6 MrCE8a 20.868 8.9736
e.m.s_0215_0001.21 Carbonic anhydrase 16.3 0.4 2.2E-02
e.m.s_0250_0003.8 Catalase cat1 294.9 59.3 6.4E-03 5.0 MrCAT1 114.906 48.407
e.m.s_0155_0004.13 Cell wall glycosyl hydrolase 330.4 26.3 7.4E-05 12.6
e.m.s_0192_0001.1 Cerato-platanin 3014.0 520.7 5.3E-03 5.8
e.m.s_0192_0003.14 Cerato-platanin 4931.3 968.5 7.2E-03 5.1
e.m.s_0192_0003.17 Cerato-platanin 7602.6 912.5 7.1E-04 8.3
e.m.s_0258_0001.1 Defense-related protein scp domain-containing protein MrPR-1 g 16778.8 1200.4 5.6E-04 14.0 MrSCP 434.018 253.661
e.m.s_0251_0004.1 Defense-related protein scp domain-containing protein MrPR-1i2 32.6 2.4 7.4E-02
e.m.s_0516_0001.1 Defense-related protein scp domain-containing protein Mr-PR-1n 499.0 13.7 2.6E-06 38.3
e.m.s_0041_0005.1 Endo-polygalacturonase 2621.4 32.4 1.4E-06 81.9 MrendoPG 911.453 592.910
e.m.s_0407_0004.2 Galactan 1,3-beta-galactosidase 37.6 52.2 2.4E-02
e.m.s_0027_0002.19 Galactose 1-epimerase 154.2 47.4 3.9E-02 3.3
e.m.s_0011_0002.91 Glycoside hydrolase family 16 protein 1276.8 232.0 5.0E-03 5.5
e.m.s_0030_0003.33 Glycoside hydrolase family 18 protein 6125.5 948.8 3.4E-03 6.5 MrGH18g 38.900 16.126
e.m.s_0044_0001.33 Glycoside hydrolase family 18 protein 527.3 56.3 7.7E-04 9.4 MrGH18d 253.832 183.817
e.m.s_0044_0001.6 Glycoside hydrolase family 18 protein 12287.0 3336.6 2.1E-02 3.7
e.m.s_0114_0002.6 Glycoside hydrolase family 18 protein 12684.6 687.6 5.1E-06 18.5 MrGH18c 151.387 51.810
e.m.s_0416_0001.5 Glycoside hydrolase family 18 protein 4897.1 266.4 3.1E-06 18.4
e.m.s_0055_0003.9 Glycoside hydrolase family 28 protein 129.2 20.9 1.3E-02 6.1
e.m.s_0067_0006.17 Glycoside hydrolase family 43 234.2 54.4 2.2E-02 4.3
e.m.s_0043_0001.2 Glycoside hydrolase family 5 protein 3971.1 188.7 1.2E-06 21.1
e.m.s_0531_0001.4 Glycoside hydrolase family 5 protein 3387.9 716.4 1.2E-02 4.7 MrGH5 69.7456 41.581
e.m.s_0351_0003.10 Glycoside hydrolase family 92 protein 2935.9 894.7 4.6E-02 3.3
e.m.s_0346_0003.2 Hydrophobin 3 431.5 65.6 1.8E-03 6.6 MrSc3P3 345.0789 196.349
e.m.s_0346_0003.1 Hydrophobin sc3-like 6461.8 471.4 3.8E-05 13.7 MrSc3P2 356.391 177.576
e.m.s_0409_0005.4 Hypothetical fad dependent oxidoreductase 566.2 119.8 1.4E-02 4.7
e.m.s_0561_0002.1 Immunomodulatory protein 7180.5 746.5 3.2E-04 9.6
e.m.s_0095_0005.5 Major facilitator superfamily multidrug-resistance 7.0 0.0 1.3E-02
e.m.s_0053_0004.6 Membrane autotransporter barrel domain protein 755.7 203.5 2.5E-02 3.7 MrBDP 5.184 1.528
e.m.s_0094_0001.8 O-methyltransferase 1131.7 488.6 8.2E-04
e.m.s_0064_0001.5 Tryptophan dimethylallyltransferase 190.8 0.9 3.2E-04 211.1 MrTRY-DMA 235.864 134.155
e.m.s_0039_0002.43 WSC domain protein 356.0 102.1 4.1E-02 3.5
e.m.s_0478_0002.2 Xylanase A 1135.8 9.0 9.3E-04 126.0 MrXYLa 171.086 77.159

Mean* is the normalized mean based on the size factor of the libraries.

Meinhardt et al.

Meinhardt et al. BMC Genomics 2014 15:164   doi:10.1186/1471-2164-15-164

Open Data