Open Access Research article

Genome and secretome analysis of the hemibiotrophic fungal pathogen, Moniliophthora roreri, which causes frosty pod rot disease of cacao: mechanisms of the biotrophic and necrotrophic phases

Lyndel W Meinhardt1*, Gustavo Gilson Lacerda Costa2, Daniela PT Thomazella3, Paulo José PL Teixeira3, Marcelo Falsarella Carazzolle2, Stephan C Schuster4, John E Carlson5, Mark J Guiltinan6, Piotr Mieczkowski7, Andrew Farmer8, Thiruvarangan Ramaraj8, Jayne Crozier9, Robert E Davis10, Jonathan Shao10, Rachel L Melnick1, Gonçalo AG Pereira3 and Bryan A Bailey1

Author Affiliations

1 Sustainable Perennial Crops Lab, USDA/ARS, Bldg 001 Rm 223 Beltsville Agricultural Research Center-West, Beltsville, MD 20705, USA

2 Centro Nacional de Processamento de Alto Desempenho em São Paulo, Universidade Estadual de Campinas, CP 6141, Campinas 13083-970, SP, Brazil

3 Laboratório de Genômica e Expressão, Departamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, Campinas 13083-970, SP, Brazil

4 Center for Infectious Disease Dynamics, Pennsylvania State University, University Park, PA 16802, USA

5 Department of Ecosystem Science and Management, Pennsylvania State University, University Park, PA 16802, USA

6 Department of Horticulture, Pennsylvania State University, University Park, PA 16802, USA

7 Department of Genetics, School of Medicine, University of North Carolina at Chapel Hill, Mary Ellen Jones, Room 921, 27599-3280 Chapel Hill, NC, USA

8 National Center of Genomic Research, 2935 Rodeo Park Drive East Santa Fe, NM 87505 Santa Fe, USA

9 CABI Bioscience UK Centre, Egham, UK

10 Molecular Plant Pathology Lab, USDA/ARS, Bldg 004 Rm 119 Beltsville Agricultural Research Center West, Beltsville, MD 20705, USA

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BMC Genomics 2014, 15:164  doi:10.1186/1471-2164-15-164

Published: 27 February 2014

Additional files

Additional file 1:

This file contains the Repeat Modeler file and a complete list of the Venn diagram intersects; A, AB, ABC and ABE discussed in the text. There is a sheet for each set and intersect and RNA-Seq data are provided for each gene in that intersect.

Format: XLSX Size: 273KB Download file

Open Data

Additional file 2:

This file contains the complete list of the 1535 secreted genes and the 125 GPI-anchor containing proteins identified in the M. roreri genome. The file contains RNA-Seq data for each gene and RT-qPCR data for a number of selected genes. The corresponding M. perniciosa homolog is also listed with the homology E-value.

Format: XLSX Size: 368KB Download file

Open Data

Additional file 3:

This file contains the complete set of RT-qPCR primers used in this study. The file provides the primer name, nucleotide sequence, M. roreri gene Id, PCR fragment size and the nucleotide sequence for each fragment.

Format: XLSX Size: 39KB Download file

Open Data