Open Access Highly Accessed Methodology article

Ultra-deep mutant spectrum profiling: improving sequencing accuracy using overlapping read pairs

Haiyin Chen-Harris*, Monica K Borucki, Clinton Torres, Tom R Slezak and Jonathan E Allen

Author Affiliations

Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, USA

For all author emails, please log on.

BMC Genomics 2013, 14:96  doi:10.1186/1471-2164-14-96

Published: 12 February 2013

Additional files

Additional file 1:Figure S1, Table S1, Table S2, Table S3, Table S4:

Figure S1 is on the effect of quality filtering on coverage. Table S1 summarizes the sequencing output. Table S2 lists the false positive rates among ORP reads after applying the variant detection model to the control sequences at their coverage level and two other hypothetical coverage levels. Table S3 and table S4 list the primers used to amplify regions of the BCV and rabies genomes, respectively. Additional discussion on the relative contribution of sequencing and PCR error rates is also included.

Format: PDF Size: 413KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data