Strategy employed to identify primary JunD targets in bone marrow derived macrophages. We performed Jund RNAi and compared whole genome expression profiling in macrophages (basal and stimulated with LPS, 100ng/ml, 8h) transfected with scrambled siRNA to those transfected with Jund siRNA. ChIP-Seq analysis was performed in WKY and WKY.LCrgn2 BMDMs (basal and stimulated with LPS, 100ng/ml, 2h). Transcripts showing a fold change > 3 in the RNAi dataset were examined for JunD/AP1 peaks.
Hull et al. BMC Genomics 2013 14:92 doi:10.1186/1471-2164-14-92