Preparation of R15 single neurons from Aplysia abdominal ganglia for genomic analysis. Aplysia central nervous system is organized into nine different ganglia. Identified neurons of abdominal ganglia are shown. Single R15 neuron was isolated from anesthetized Aplysia and immediately stored in Trizol at -80°C. Total RNA was isolated using standard Trizol RNA isolation method and amplified using MessageAmp™ II aRNA Amplification Kit. Quality control and quantification of freshly isolated CNS was performed using a Bioanalyzer and by spectrophotometry.
Kadakkuzha et al. BMC Genomics 2013 14:880 doi:10.1186/1471-2164-14-880