Open Access Highly Accessed Research article

Carbohydrate utilization and metabolism is highly differentiated in Agaricus bisporus

Aleksandrina Patyshakuliyeva1, Edita Jurak2, Annegret Kohler3, Adam Baker4, Evy Battaglia15, Wouter de Bruijn2, Kerry S Burton6, Michael P Challen7, Pedro M Coutinho8, Daniel C Eastwood9, Birgit S Gruben15, Miia R Mäkelä10, Francis Martin3, Marina Nadal5, Joost van den Brink1, Ad Wiebenga1, Miaomiao Zhou1, Bernard Henrissat8, Mirjam Kabel2, Harry Gruppen2 and Ronald P de Vries15*

Author Affiliations

1 CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands

2 Wageningen University, Laboratory of Food Chemistry, Bomenweg 2, 6703 HD Wageningen, The Netherlands

3 INRA, UMR1136 INRA/UHP, Interactions Arbres/ Micro-organismes, Centre de Nancy, Champenoux 54280, France

4 University of Warwick, Warwick, CV35 9EF, Wellesbourne, UK

5 Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

6 East Malling Research, New Road, East Malling, Kent ME19 6BJ, UK

7 Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK

8 UMR 6098 CNRS–Universités Aix-Marseille I and II, Marseille Cedex 9 13288, France

9 College of Science, University of Swansea, Singleton Park, Swansea SA2 8PP, UK

10 Department of Food and Environmental Sciences, University of Helsinki, P. O. Box 56, 00014 Helsinki, Finland

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BMC Genomics 2013, 14:663  doi:10.1186/1471-2164-14-663

Published: 30 September 2013

Abstract

Background

Agaricus bisporus is commercially grown on compost, in which the available carbon sources consist mainly of plant-derived polysaccharides that are built out of various different constituent monosaccharides. The major constituent monosaccharides of these polysaccharides are glucose, xylose, and arabinose, while smaller amounts of galactose, glucuronic acid, rhamnose and mannose are also present.

Results

In this study, genes encoding putative enzymes from carbon metabolism were identified and their expression was studied in different growth stages of A. bisporus. We correlated the expression of genes encoding plant and fungal polysaccharide modifying enzymes identified in the A. bisporus genome to the soluble carbohydrates and the composition of mycelium grown compost, casing layer and fruiting bodies.

Conclusions

The compost grown vegetative mycelium of A. bisporus consumes a wide variety of monosaccharides. However, in fruiting bodies only hexose catabolism occurs, and no accumulation of other sugars was observed. This suggests that only hexoses or their conversion products are transported from the vegetative mycelium to the fruiting body, while the other sugars likely provide energy for growth and maintenance of the vegetative mycelium. Clear correlations were found between expression of the genes and composition of carbohydrates. Genes encoding plant cell wall polysaccharide degrading enzymes were mainly expressed in compost-grown mycelium, and largely absent in fruiting bodies. In contrast, genes encoding fungal cell wall polysaccharide modifying enzymes were expressed in both fruiting bodies and vegetative mycelium, but different gene sets were expressed in these samples.