Upregulation of miR-296-3p and miR-298-5p reduces αTC1-6 resistance to apoptosis induced by cytokines. Annexin V flow cytometric analysis of apoptosis in αTC1-6 transiently transfected with scrambled oligonucleotides (NC, Negative Control), mimics of miR-296-3p, miR-298-5p, a mix of both, and treated with IFN-γ, IL-1β, TNF-α for 6, 24, 48 h. The y-axis represents the percentage of apoptotic cells; the x-axis represents the four experimental conditions [(i)scramble-transfected cells; (ii) cells transfected with mimics of miR-296-3p; (iii) cells transfected with mimics of miR-298-5p; (iv) cells transfected with a mix of both] assayed at the three time-points. Data are presented as mean ± S.D. of three independent experiments (n = 3). All the possible pairwise comparisons were performed among the four different experimental conditions within each time point: significant differences have been assessed through Tukey HSD post-hoc one-way ANOVA test (** p-value < 0.01). At 24 h PT, αTC1-6 transfected with mimics of miR-298-5p show a highly significant increase of the number of apoptotic cells with respect to scramble-transfected control; at the same time point, in αTC1-6 transfected with mimics of both miR-296-3p and miR-298-5p a highly significant increase of the number of apoptotic cells is detected with respect to all the other experimental conditions.
Barbagallo et al. BMC Genomics 2013 14:62 doi:10.1186/1471-2164-14-62