Expression of miR-296-3p and miR-298-5p in αTC1-6 and βTC1. (A) Real-time PCR amplification plot of miR-296-3p and miR-298-5p in αTC1-6 and βTC1 at steady state. Note that both miRNAs are not expressed in βTC1. Plot image is representative of three independent experiments (n = 3). (B) Gene expression fold changes of miR-296-3p and miR-298-5p in αTC1-6 at 6, 12, 24, 48 h PT, relative to matched untreated cells. MiRNAs expression was measured by quantitative real-time PCR. MiR-26a was used as endogenous control. Data are reported as mean ± S.D. of three independent experiments (n = 3). * p-value < 0.05; ** p-value < 0.01 (Student’s t-test, Bonferroni correction).
Barbagallo et al. BMC Genomics 2013 14:62 doi:10.1186/1471-2164-14-62