Table 1

Gene features in three Puccinia triticina BAC clones and their alignment to other sequenced Basidiomycetes, P. graminis tritici (Pgt), Melampsora larici-populina (Mlp), and Ustilago maydis (Um)
P. triticina PgtGencxxe Feature* Pgt protein Mlp Um BLASTX
ORF identities gene feature** gene feature Annotation
1 F16-1 PGTG_12990 54% 35.90208/11 - Similar to Uhrf1
1 F16-2 PGTG_13012 60% 104.11397 Um_00786 Hypothetical protein
1 F16-3 PGTG_13013 63% 74. 94858 - Similar to esterase
1 F16-4 PGTG_13016 37% - - Predicted protein
PGTG_18731 64%
1 F16-5 PGTG_13018 67% 49.39112 Um_02725 Similar to molybdopterin synthase sulpherylase
PGTG_18732 68%
1 F16-6 PGTG_13021 68% 74.73436 - Hypothetical protein
PGTG_18735 64%
1 F16-7 PGTG_13023 56% 74.94864 Um_05085 RAD18
PGTG_18741 56%
1 F16-8 PGTG_13024 64% 74.000024 - cystein rich SCP-like extracellular protein
PGTG_18744 65%
1 F16-9 PGTG_13026 87% 74.50754/28052 Um_00594 Similar to pyruvate dehydrogenase complex
PGTG_18746 86%
HSP02-1 PGTG_3730/1 79% 22.87674 Um_00736 Conserved protein
HSP02-2 PGTG_6672 48% 68.40205 Um_04270 Aspartyl-tRNA synthetase
HSP02-3 PGTG_3706 30% - - -
HSP02-4 PGTG_3708 69% 2.70587 Um_01555 Mlp HESP-379
HSP02-5 PGTG_3709 83% 2.70587 Um_01555 Mlp HESP-379
HSP02-6 PGTG_3727 100% 2.76428 Um_00703 G-protein beta subunit
HSP02-7 PGTG_3728 68% 2.115002 Um_03486 Nucleotide-binding protein 2
HSP02-8 PGTG_3729 82% 2.46419 Um_05743 Pre-mRNA splicing factor ATP-dependent RNA helicase PRP16
HSP02-9 PGTG_3730 87% 10.115914 Um_04551 Similar to cyclin Ctk2
HSP04-1 PGTG_16978 36% - - Predicted protein
HSP04-2 PGTG_16976 73% 27.88522 Um_00639 Nucleoporin-like
HSP04-3 PGTG_10949 95% 47.72927 Um_02479 60S ribosomal protein
HSP04-4 PGTG_02586 33% 27.88520 - Heat shock protein 90
HSP04-5 PGTG_14539 41% - - Predicted protein
HSP04-6 PGTG_17549 26% - - Predicted secreted protein
HSP04-7 PGTG_17549 60% - - Predicted secreted protein
HSP04-8 PGTG_17547/8 76% 16.85997 - Uf-HSP42c
HSP04-9 PGTG_17547/8 71% 16.85997 - Uf-HSP42c
HSP04-10 PGTG_05205 82% 22.48630 - Integral membrane protein
HSP04-11 PGTG_17545 52% - Um_00662 Predicted protein
HSP04-12 PGTG_17544 83% 23.87824 Um_03820 Vacuolar sorting protein PEP5
HSP04-13 PGTG_17543 62% 23.72100 Um_02189 Hypothetical protein
HSP04-14 PGTG_17537/8 38% - - Predicted protein

* Pgt gene features indicate gene nUmber assigned by the Broad institute during assembly (http://www.broadinstitute.org/annotation/genome/puccinia_group/ webcite verified September 28, 2012). Two nUmbers indicate different scaffolds, as highlighted in Figure 1.

** Mlp gene features are indicated by scaffold nUmber; gene nUmber as assigned by the Joint Genome Initiative at the Department of Energy (http://genomeportal.jgi-psf.org/Mellp1/Mellp1.home.html webcite verified September 28, 2012).

Fellers et al.

Fellers et al. BMC Genomics 2013 14:60   doi:10.1186/1471-2164-14-60

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