Additional file 3.
Figure S1. qPCR to evaluate the knock-down of TcABC transcripts by RNAi. dsRNA specific for different TcABC genes was injected into penultimate instar larvae (PL), pre-pupae (PP) or adults (A). Total RNA was prepared three days after the injections. Mean normalized expression values were determined by qPCR with primers specific to the indicated target gene by comparing CT values of the respective target gene and the reference gene TcRPS6. As a control, the mean normalized expression after injection of dsRNA specific for the TcABC genes was compared with that of insects injected with dsRNA specific for TcVer.
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Broehan et al. BMC Genomics 2013 14:6 doi:10.1186/1471-2164-14-6