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Open Access Highly Accessed Research article

Functional analysis of the ATP-binding cassette (ABC) transporter gene family of Tribolium castaneum

Gunnar Broehan1, Tobias Kroeger1, Marcé Lorenzen2 and Hans Merzendorfer1*

Author affiliations

1 Department of Biology/Chemistry, Animal Physiology, University of Osnabrück, Osnabrück, 49069, Germany

2 Department of Entomology, North Carolina State University, Raleigh, NC, 27695, USA

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Citation and License

BMC Genomics 2013, 14:6  doi:10.1186/1471-2164-14-6

Published: 16 January 2013

Abstract

Background

The ATP-binding cassette (ABC) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. Most are integral membrane proteins that transport a broad spectrum of substrates across lipid membranes. In insects, ABC transporters are of special interest because of their role in insecticide resistance.

Results

We have identified 73 ABC transporter genes in the genome of T. castaneum, which group into eight subfamilies (ABCA-H). This coleopteran ABC family is significantly larger than those reported for insects in other taxonomic groups. Phylogenetic analysis revealed that this increase is due to gene expansion within a single clade of subfamily ABCC. We performed an RNA interference (RNAi) screen to study the function of ABC transporters during development. In ten cases, injection of double-stranded RNA (dsRNA) into larvae caused developmental phenotypes, which included growth arrest and localized melanization, eye pigmentation defects, abnormal cuticle formation, egg-laying and egg-hatching defects, and mortality due to abortive molting and desiccation. Some of the ABC transporters we studied in closer detail to examine their role in lipid, ecdysteroid and eye pigment transport.

Conclusions

The results from our study provide new insights into the physiological function of ABC transporters in T. castaneum, and may help to establish new target sites for insect control.

Keywords:
ABC transporter; Coleoptera; dsRNA; Genome; RNA interference; Red flour beetle; Tribolium castaneum