Open Access Research article

Development of a high density 600K SNP genotyping array for chicken

Andreas Kranis1*, Almas A Gheyas2, Clarissa Boschiero2, Frances Turner2, Le Yu2, Sarah Smith2, Richard Talbot2, Ali Pirani3, Fiona Brew3, Pete Kaiser2, Paul M Hocking2, Mark Fife4, Nigel Salmon4, Janet Fulton5, Tim M Strom6, Georg Haberer7, Steffen Weigend8, Rudolf Preisinger9, Mahmood Gholami10, Saber Qanbari10, Henner Simianer10, Kellie A Watson1, John A Woolliams2 and David W Burt2*

Author affiliations

1 Aviagen Ltd, Midlothian, UK

2 The Roslin Institute, University of Edinburgh, Midlothian, UK

3 Affymetrix, Santa Clara, CA, USA

4 The Pirbright Institute, Compton Laboratory, Compton, UK

5 Hy-Line International, Dallas Center, IA, USA

6 Institute of Human Genetics, Helmholtz Zentrum, 85764, Neuherberg, Germany

7 Institute of Bioinformatics and System Biology, Helmholtz Zentrum München, 85764, Neuherberg, Germany

8 Institute of Farm Animal Genetics, Friedrich Loeffler Institut, Neustadt-Mariensee, Germany

9 Lohmann Tierzucht GmbH, Cuxhaven, Germany

10 Animal Breeding and Genetics Group, Georg-August-University Goettingen, Goettingen, Germany

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Citation and License

BMC Genomics 2013, 14:59  doi:10.1186/1471-2164-14-59

Published: 28 January 2013



High density (HD) SNP genotyping arrays are an important tool for genetic analyses of animals and plants. Although the chicken is one of the most important farm animals, no HD array is yet available for high resolution genetic analysis of this species.


We report here the development of a 600 K Affymetrix® Axiom® HD genotyping array designed using SNPs segregating in a wide variety of chicken populations. In order to generate a large catalogue of segregating SNPs, we re-sequenced 243 chickens from 24 chicken lines derived from diverse sources (experimental, commercial broiler and layer lines) by pooling 10–15 samples within each line. About 139 million (M) putative SNPs were detected by mapping sequence reads to the new reference genome (Gallus_gallus_4.0) of which ~78 M appeared to be segregating in different lines. Using criteria such as high SNP-quality score, acceptable design scores predicting high conversion performance in the final array and uniformity of distribution across the genome, we selected ~1.8 M SNPs for validation through genotyping on an independent set of samples (n = 282). About 64% of the SNPs were polymorphic with high call rates (>98%), good cluster separation and stable Mendelian inheritance. Polymorphic SNPs were further analysed for their population characteristics and genomic effects. SNPs with extreme breach of Hardy-Weinberg equilibrium (P < 0.00001) were excluded from the panel. The final array, designed on the basis of these analyses, consists of 580,954 SNPs and includes 21,534 coding variants. SNPs were selected to achieve an essentially uniform distribution based on genetic map distance for both broiler and layer lines. Due to a lower extent of LD in broilers compared to layers, as reported in previous studies, the ratio of broiler and layer SNPs in the array was kept as 3:2. The final panel was shown to genotype a wide range of samples including broilers and layers with over 100 K to 450 K informative SNPs per line. A principal component analysis was used to demonstrate the ability of the array to detect the expected population structure which is an important pre-investigation step for many genome-wide analyses.


This Affymetrix® Axiom® array is the first SNP genotyping array for chicken that has been made commercially available to the public as a product. This array is expected to find widespread usage both in research and commercial application such as in genomic selection, genome-wide association studies, selection signature analyses, fine mapping of QTLs and detection of copy number variants.

Genotyping array; Chicken; SNP