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Open Access Research article

The impact of breed and tissue compartment on the response of pig macrophages to lipopolysaccharide

Ronan Kapetanovic1, Lynsey Fairbairn2, Alison Downing1, Dario Beraldi3, David P Sester4, Tom C Freeman1, Christopher K Tuggle5, Alan L Archibald1 and David A Hume1*

Author Affiliations

1 The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, Edinburgh EH25 9RG, United Kingdom

2 Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, 81679, Munich, Germany

3 Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK

4 Innate Immunity Laboratory, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland QLD 4072, Australia

5 Department of Animal Science, Iowa State University, Ames, IA 50011, USA

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BMC Genomics 2013, 14:581  doi:10.1186/1471-2164-14-581

Published: 28 August 2013

Abstract

Background

The draft genome of the domestic pig (Sus scrofa) has recently been published permitting refined analysis of the transcriptome. Pig breeds have been reported to differ in their resistance to infectious disease. In this study we examine whether there are corresponding differences in gene expression in innate immune cells

Results

We demonstrate that macrophages can be harvested from three different compartments of the pig (lungs, blood and bone-marrow), cryopreserved and subsequently recovered and differentiated in CSF-1. We have performed surface marker analysis and gene expression profiling on macrophages from these compartments, comparing twenty-five animals from five different breeds and their response to lipopolysaccharide. The results provide a clear distinction between alveolar macrophages (AM) and monocyte-derived (MDM) and bone-marrow-derived macrophages (BMDM). In particular, the lung macrophages express the growth factor, FLT1 and its ligand, VEGFA at high levels, suggesting a distinct pathway of growth regulation. Relatively few genes showed breed-specific differential expression, notably CXCR2 and CD302 in alveolar macrophages. In contrast, there was substantial inter-individual variation between pigs within breeds, mostly affecting genes annotated as being involved in immune responses.

Conclusions

Pig macrophages more closely resemble human, than mouse, in their set of macrophage-expressed and LPS-inducible genes. Future research will address whether inter-individual variation in macrophage gene expression is heritable, and might form the basis for selective breeding for disease resistance.

Keywords:
Pig; Macrophages; Microarray; Breed; Lipopolysaccharide