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Identifying potential RNAi targets in grain aphid (Sitobion avenae F.) based on transcriptome profiling of its alimentary canal after feeding on wheat plants

Min Zhang1, Yuwen Zhou2, Hui Wang1, Huw Dylan Jones3, Qiang Gao2, Dahai Wang1, Youzhi Ma1* and Lanqin Xia1*

Author Affiliations

1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China

2 Beijing Genomics Institute-Shenzhen (BGI-Shenzhen), Chinese Academy of Sciences, Shenzhen 518083, China

3 Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK

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BMC Genomics 2013, 14:560  doi:10.1186/1471-2164-14-560

Published: 16 August 2013

Additional files

Additional file 1:

Summary of 5490 DEGs and their annotations. The relative expression levels of DEGs and their annotations in the nr, COG, GO, and KEGG public databases were summarized. Among the 5490 differentially expressed genes, only 3805 were annotated in one of the above public databases.

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Additional file 2:

GO terms enriched in the differentially expressed genes. The results were summarized in three main categories: biological process, cellular component and molecular function. GO analysis showed that three molecular functions were found to be enriched at p < 0.05 level, and eight biological processes were significantly enriched at p < 0.05 level in the alimentary canals of grain aphid upon feeding on wheat plants.

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Additional file 3:

KEGG pathways enriched in the differentially expressed genes. KEGG pathway analysis showed that 420 unigenes which are involved in ‘Metabolic pathways’ showed differentially expressed manner, accounting for 18.09% of total DEGs (2322) with pathway annotation.

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